Isolation and expression analysis of cellulose synthase genes in Chinese fir （<em>Cunninghamia lanceolata</em>）

PANG Jing; TONG Zaikang; HUANG Huahong; LIN Erpei; LIU Qiongyao

doi:10.11833/j.issn.2095-0756.2015.01.006

Volume 32 Issue 1

Jan. 2015

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PANG Jing, TONG Zaikang, HUANG Huahong, LIN Erpei, LIU Qiongyao. Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）[J]. Journal of Zhejiang A&F University, 2015, 32(1): 40-46. doi: 10.11833/j.issn.2095-0756.2015.01.006

Citation:

PANG Jing, TONG Zaikang, HUANG Huahong, LIN Erpei, LIU Qiongyao. Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）[J]. Journal of Zhejiang A&F University, 2015, 32(1): 40-46. doi: 10.11833/j.issn.2095-0756.2015.01.006

Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）

doi: 10.11833/j.issn.2095-0756.2015.01.006

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Received Date: 2013-12-30
Rev Recd Date: 2013-05-16
Publish Date: 2015-02-20

Abstract

In this study, two full-length cDNAs encoding cellulose synthase （CESA） were isolated from Chinese fir （Cunninghamia lanceolata） using RT-PCR and RACE technology, and their sequence lengths were 3 235 bp and 3 876 bp, respectively. They were named ClCesA1 and ClCesA2, and the corresponding GenBank accession numbers were JQ844574 and JQ844575 respectively. The encoded proteins are composed of 992 and 1 092 amino acids, with theoretical molecular weight of 111 845.3 and 123 105.7 D, whose isoelectric points are 6.04 and 6.65, respectively. Amino acid sequences contain typical motifs of plant CESA proteins: a ring finger domain, two class specific regions CSRI and CSRII, two conserved regions CRI and CRII, cellulose synthase substrate binding domain D, D, D, QVLRW, and eight transmembrane regions. The expression analysis showed that ClCesA1 and ClCesA2 genes exhibited the high transcript abundance in stem, the corresponding expression levels in mature xylem were higher than that in cortex. The two genes had the lower expression levels in root and leaf. The further expression analysis was conducted using the dwarf Chinese fir with the specific wood properties. The results showed that the expression levels of ClCesA1 and ClCesA2 in the xylem of normal fir were about 2-12 times than that of the dwarf. Two ClCesA genes may be play important roles in the wood formation of Chinese fir. ［Ch, 5 fig. 19 ref.］
- forest tree breeding,
- Cunninghamia lanceolata,
- CesA gene,
- cloning,
- sequence analysis,
- expression analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Received Date: 2013-12-30

Rev Recd Date: 2013-05-16

Publish Date: 2015-02-20

Key words:

Abstract: In this study, two full-length cDNAs encoding cellulose synthase （CESA） were isolated from Chinese fir （Cunninghamia lanceolata） using RT-PCR and RACE technology, and their sequence lengths were 3 235 bp and 3 876 bp, respectively. They were named ClCesA1 and ClCesA2, and the corresponding GenBank accession numbers were JQ844574 and JQ844575 respectively. The encoded proteins are composed of 992 and 1 092 amino acids, with theoretical molecular weight of 111 845.3 and 123 105.7 D, whose isoelectric points are 6.04 and 6.65, respectively. Amino acid sequences contain typical motifs of plant CESA proteins: a ring finger domain, two class specific regions CSRI and CSRII, two conserved regions CRI and CRII, cellulose synthase substrate binding domain D, D, D, QVLRW, and eight transmembrane regions. The expression analysis showed that ClCesA1 and ClCesA2 genes exhibited the high transcript abundance in stem, the corresponding expression levels in mature xylem were higher than that in cortex. The two genes had the lower expression levels in root and leaf. The further expression analysis was conducted using the dwarf Chinese fir with the specific wood properties. The results showed that the expression levels of ClCesA1 and ClCesA2 in the xylem of normal fir were about 2-12 times than that of the dwarf. Two ClCesA genes may be play important roles in the wood formation of Chinese fir. ［Ch, 5 fig. 19 ref.］

Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）

doi: 10.11833/j.issn.2095-0756.2015.01.006

Abstract

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Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）

doi: 10.11833/j.issn.2095-0756.2015.01.006

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）

doi: 10.11833/j.issn.2095-0756.2015.01.006

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通讯作者: 陈斌, bchen63@163.com

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Isolation and expression analysis of cellulose synthase genes in Chinese fir （Cunninghamia lanceolata）

doi: 10.11833/j.issn.2095-0756.2015.01.006

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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