Genetic transformation of <em>Myrica ru</em>bra mediated by <em>Agrobacterium rhizogenes</em>

HE Xin-hua; CHEN Li-geng; GUO Chang-lu

Volume 24 Issue 4

Oct. 2016

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Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(4): 433-436

HE Xin-hua, CHEN Li-geng, GUO Chang-lu. Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes[J]. Journal of Zhejiang A&F University, 2007, 24(4): 433-436.

Citation:

HE Xin-hua, CHEN Li-geng, GUO Chang-lu. Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes[J]. Journal of Zhejiang A&F University, 2007, 24(4): 433-436.

Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes

1.
Department of Horticulture, Guangxi University, Nanning 530004, Guangxi, China;
2.
Department of Horticulture, Zhejiang University, Hangzhou 310029, Zhejiang, China;
3.
School of the Ocean, Harbin Institute of Technology, Weihai 264209, Shandong, China

Funds:

国家自然科学基金资助项目(30560007);浙江省自然科学基金资助项目(302362)

Received Date: 2006-11-03
Rev Recd Date: 2007-04-09
Publish Date: 2007-08-20

Abstract

Myrica rubra(China bayberry) is a particular fruit tree to China. It is also a ideal tree species of afforestation. The purpose is to establish a genetic transformation system of Myrica rubra mediated with Agrobacterium rhizogenes. Cotyledons, leaves, and stem segments of M. rubra Dongkui and M. rubra Boji' were infected with A. rhizogenes R15834 containing a pDC202 vector carriing the LEAFY gene, which controls floral meristem, cultured in 1/2Murashige and Skoog(MS)orMSmedium. Results showed thatonly less than10%of the cotyledons of M. rubra Dongkui' induced hairy roots. Hairy roots were produced from new seeds of M. rubra Dongkui' more easily than old seeds, and the cotyledons grew hairy roots on the 1/2 MS media more readily than the MS media. By PCR and Southern hybridization test, it was proved that the LEAFY gene of Arabidopsis thaliana was successfully transferred and integrated into a nucleargenome through cotyledons of M. rubra Dongkui'.[Ch, 2 fig. 10 ref.]
- cash forest,
- Myica rubra,
- genetic transformation,
- Agrobacterium rhizogenes

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes

1. Department of Horticulture, Guangxi University, Nanning 530004, Guangxi, China;

2. Department of Horticulture, Zhejiang University, Hangzhou 310029, Zhejiang, China;

3. School of the Ocean, Harbin Institute of Technology, Weihai 264209, Shandong, China

Funds:

国家自然科学基金资助项目(30560007);浙江省自然科学基金资助项目(302362)

Received Date: 2006-11-03

Rev Recd Date: 2007-04-09

Publish Date: 2007-08-20

Key words:

Abstract: Myrica rubra(China bayberry) is a particular fruit tree to China. It is also a ideal tree species of afforestation. The purpose is to establish a genetic transformation system of Myrica rubra mediated with Agrobacterium rhizogenes. Cotyledons, leaves, and stem segments of M. rubra Dongkui and M. rubra Boji' were infected with A. rhizogenes R15834 containing a pDC202 vector carriing the LEAFY gene, which controls floral meristem, cultured in 1/2Murashige and Skoog(MS)orMSmedium. Results showed thatonly less than10%of the cotyledons of M. rubra Dongkui' induced hairy roots. Hairy roots were produced from new seeds of M. rubra Dongkui' more easily than old seeds, and the cotyledons grew hairy roots on the 1/2 MS media more readily than the MS media. By PCR and Southern hybridization test, it was proved that the LEAFY gene of Arabidopsis thaliana was successfully transferred and integrated into a nucleargenome through cotyledons of M. rubra Dongkui'.[Ch, 2 fig. 10 ref.]

Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes

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通讯作者: 陈斌, bchen63@163.com

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Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes

1. Department of Horticulture, Guangxi University, Nanning 530004, Guangxi, China;

2. Department of Horticulture, Zhejiang University, Hangzhou 310029, Zhejiang, China;

3. School of the Ocean, Harbin Institute of Technology, Weihai 264209, Shandong, China

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Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes

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通讯作者: 陈斌, bchen63@163.com

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Genetic transformation of Myrica rubra mediated by Agrobacterium rhizogenes

1. Department of Horticulture, Guangxi University, Nanning 530004, Guangxi, China; 2. Department of Horticulture, Zhejiang University, Hangzhou 310029, Zhejiang, China; 3. School of the Ocean, Harbin Institute of Technology, Weihai 264209, Shandong, China

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1. Department of Horticulture, Guangxi University, Nanning 530004, Guangxi, China;

2. Department of Horticulture, Zhejiang University, Hangzhou 310029, Zhejiang, China;

3. School of the Ocean, Harbin Institute of Technology, Weihai 264209, Shandong, China