RNA-mediated gene silence in plants and its application

AI Jian-guo; YANG Yong

Volume 22 Issue 1

Feb. 2017

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2005 > 22(1): 123-128

AI Jian-guo, YANG Yong. RNA-mediated gene silence in plants and its application[J]. Journal of Zhejiang A&F University, 2005, 22(1): 123-128.

Citation:

AI Jian-guo, YANG Yong. RNA-mediated gene silence in plants and its application[J]. Journal of Zhejiang A&F University, 2005, 22(1): 123-128.

RNA-mediated gene silence in plants and its application

AI Jian-guo^{1
,
,},
YANG Yong²

1.
.School of Life Sciences, Zhejiang Forestry College , Lin,an 311300 , Zhejiang , China;
2.
.State Key Laboratory of Plant Physiology and Biochemistry , College of Life Sciences , Zhejiang University , Hangzhou 310029 , Zhejiang , China

Received Date: 2004-06-03
Rev Recd Date: 2004-09-10
Publish Date: 2005-02-20

Abstract

Gene silencing , commonly occurring in a variety of organisms , is one of the important regulatory mechanisms for gene expression .Recent studies have revealed that in plants siRNA (small interference RNA)are associated with gene silencing which includes PTGS (post-transcriptional gene silencing)and TGS (transcriptional gene silencing).The biotechnology of the RNA-mediated gene silencing has provided new avenues for highthroughput reverse genetic approaches to determine the biochemical metabolism pathways and gene functions in plants .This paper briefly introduces the molecular mechanisms of RdDM (RNA-dependent DNA methylation)and RNAi (RNA interference)and the applications in plant genetic engineering .[ Ch , 1 fig .31 ref .]
- gene silencing,
- DNA methylation,
- RNAi,
- dsRNA,
- transgene

Relative Article

[1]	ZHOU Peina, DANG Jingjie, SHAO Yongfang, SHI Zunrui, ZHANG Lin, LIU Chanchan, WU Qi’nan. Genome-wide identification and expression analysis of HD-Zip gene family in Schizonepeta tenuifolia . Journal of Zhejiang A&F University, 2023, 40(1): 12-21. doi: 10.11833/j.issn.2095-0756.20220390
[2]	WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis . Journal of Zhejiang A&F University, 2022, 39(3): 486-494. doi: 10.11833/j.issn.2095-0756.20210471
[3]	GUO Yakun, ZHAO Yanqiu, DU Juan, LU Mengzhu. Effects of DNA topoisomerase PagTOP2b on growth and development of poplar ‘84K’ . Journal of Zhejiang A&F University, 2022, 39(6): 1155-1162. doi: 10.11833/j.issn.2095-0756.20220372
[4]	RUAN Shiyu, ZHANG Zhijun, CHEN Jialu, MA Ruifang, ZHU Fengxiao, LIU Xiaoyu. Genome identification and expression analysis of GRF gene family in Phyllostachys edulis . Journal of Zhejiang A&F University, 2021, 38(4): 792-801. doi: 10.11833/j.issn.2095-0756.20200544
[5]	LI Yutong, HE Xinlong, PENG Yifan, HU Xieke, WANG Yuhang, ZHAO Ting, WU Shengchun, LIANG Peng. Influence of algal derived dissolved organic matter on mercury methylation in water . Journal of Zhejiang A&F University, 2021, 38(2): 362-368. doi: 10.11833/j.issn.2095-0756.20200146
[6]	LU Yaping, ZHOU Mingbing. On transposon silencing and DNA methylation . Journal of Zhejiang A&F University, 2021, 38(3): 634-643. doi: 10.11833/j.issn.2095-0756.20200338
[7]	CHEN Wenchong, JIA Ning, DONG Ang, YAN Daoliang, ZENG Yanru. A protocol for methylation-sensitive amplified polymorphism markers and its application to a methylation analysis in Carya cathayensis . Journal of Zhejiang A&F University, 2019, 36(3): 468-478. doi: 10.11833/j.issn.2095-0756.2019.03.006
[8]	FU Jianxin, ZHANG Chao, WANG Yiguang, ZHAO Hongbo. Reference gene selection for quantitative real-time polymerase chain reaction (qRT-PCR) normalization in the gene expression of sweet osmanthus tissues . Journal of Zhejiang A&F University, 2016, 33(5): 727-733. doi: 10.11833/j.issn.2095-0756.2016.05.001
[9]	ZHANG Liqing, CHEN Xia, HU Haichao, ZHANG Junting, GUAN Huiqian, LIU Qingpo. miR3979-mediated arsenite tolerance in rice . Journal of Zhejiang A&F University, 2016, 33(4): 571-580. doi: 10.11833/j.issn.2095-0756.2016.04.004
[10]	LIU Panfeng, DU Lanying, WU Min, HUANG Haiyan, DU Hongyan. Cloning and sequence of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase gene cDNA from Eucommia ulmoides . Journal of Zhejiang A&F University, 2014, 31(3): 410-416. doi: 10.11833/j.issn.2095-0756.2014.03.013
[11]	LI Shunfu, HU Hengkang, ZHANG Qiulu, XU Yanling, ZHANG Qixiang. ISSR analysis of DNA methylation variety on immature embryos of Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(4): 521-527. doi: 10.11833/j.issn.2095-0756.2014.04.005
[12]	DU Xue, LI Jinjun, LU Lizhi, ZHAO Ayong. Research progresses on cholesterol 7α-hydroxylase gene （CYP7A1） . Journal of Zhejiang A&F University, 2013, 30(5): 755-760. doi: 10.11833/j.issn.2095-0756.2013.05.019
[13]	JIANG Xiao-ling, HUANG Qiu-xian, LI Hong, ZHAO Jia-ping. A review of advances in plant thaumatin-like proteins . Journal of Zhejiang A&F University, 2012, 29(2): 279-287. doi: 10.11833/j.issn.2095-0756.2012.02.019
[14]	TIAN Min, GONG Mao-jiang, XU Xiao-yan, WANG Cai-xia. Research progress on gene regulation in the flower development of orchid . Journal of Zhejiang A&F University, 2011, 28(3): 494-499. doi: 10.11833/j.issn.2095-0756.2011.03.023
[15]	WANG Yi, GUO Xiao-qin, LOU Yong-feng, YANG Hai-yun, LIN Xin-chun, FANG Wei. Cloning and expression of polyphenol oxidase （PPO） in Phyllostachys nigra . Journal of Zhejiang A&F University, 2010, 27(5): 781-785. doi: 10.11833/j.issn.2095-0756.2010.05.023
[16]	SONG Hong－gai, JIANG Jing, QIAO Gui－rong, YANG Ye, ZHOU Jing, PAN Luan－yin, ZHUO Ren－ying. Plant stress－resistant gene cloning for Na and Cd using an INVSc1 yeast . Journal of Zhejiang A&F University, 2010, 27(6): 890-895. doi: 10.11833/j.issn.2095-0756.2010.06.014
[17]	GUAN Yu, ZHANG Xiang-yun, XU Qun-fang, WU Zhi-song, GU Guang-tong, NIE Wen. Hardy-Weinberg equilibrium testing and estimating of allele frequency . Journal of Zhejiang A&F University, 2009, 26(1): 122-126.
[18]	QIAOGui-rong, LUANWei-jiang, PANHong-wei, ZHUO Ren-ying. The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems . Journal of Zhejiang A&F University, 2007, 24(2): 140-144.
[19]	XIE Yi-qing, LI Zhi-zhen, HUANG Ru-zhu, XIAO Xiang-xi, WANG Zhi-jie. Comparison of methods of extracting genomic DNA from Betula luminifera . Journal of Zhejiang A&F University, 2006, 23(6): 664-668.
[20]	YU Shu-quan, LIU Jun, FU Da-rong, LIU Da-jian, LIU You-quan. Characteristics of Tacamachaca genes in the Western Sichuan Plateau . Journal of Zhejiang A&F University, 2003, 20(1): 27-31.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(1028) PDF downloads(304) Cited by()

Proportional views

RNA-mediated gene silence in plants and its application

1. .School of Life Sciences, Zhejiang Forestry College , Lin,an 311300 , Zhejiang , China;

2. .State Key Laboratory of Plant Physiology and Biochemistry , College of Life Sciences , Zhejiang University , Hangzhou 310029 , Zhejiang , China

Received Date: 2004-06-03

Rev Recd Date: 2004-09-10

Publish Date: 2005-02-20

Key words:

Abstract: Gene silencing , commonly occurring in a variety of organisms , is one of the important regulatory mechanisms for gene expression .Recent studies have revealed that in plants siRNA (small interference RNA)are associated with gene silencing which includes PTGS (post-transcriptional gene silencing)and TGS (transcriptional gene silencing).The biotechnology of the RNA-mediated gene silencing has provided new avenues for highthroughput reverse genetic approaches to determine the biochemical metabolism pathways and gene functions in plants .This paper briefly introduces the molecular mechanisms of RdDM (RNA-dependent DNA methylation)and RNAi (RNA interference)and the applications in plant genetic engineering .[ Ch , 1 fig .31 ref .]

RNA-mediated gene silence in plants and its application

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views