The optimal reaction system of RAPD in <em>Carya cathayensis</em>

WANG Zheng-jia; HUANG Jian-qin; GUO Chuan-you; YANG Ping; WANG Hua-fang

Volume 20 Issue 4

Jul. 2017

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WANG Zheng-jia, HUANG Jian-qin, GUO Chuan-you, YANG Ping, WANG Hua-fang. The optimal reaction system of RAPD in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2003, 20(4): 429-433.

Citation:

WANG Zheng-jia, HUANG Jian-qin, GUO Chuan-you, YANG Ping, WANG Hua-fang. The optimal reaction system of RAPD in Carya cathayensis[J]. Journal of Zhejiang A&F University, 2003, 20(4): 429-433.

The optimal reaction system of RAPD in Carya cathayensis

1.
School of Biological Science and Biotechnology , Beijing Forestry University , Beijing 10083, China;
2.
School of Life Sciences, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
3.
College of Forest Resources and Environment , Nanjing Forestry University , Nanjing 210037, Jiangsu, China

Funds:

浙江省科学技术厅重大招标项目(0211025); 浙江省自然科学基金重大项目(ZA0208); 杭州市科技局重大攻关项目 (2002112A12)

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Author Bio:
王正加(1972-), 男, 浙江三门人, 讲师, 从事植物生物技术研究。E-mail: wzhj21@163.com
Corresponding author: 王正加(1972-), 男, 浙江三门人, 讲师, 从事植物生物技术研究。
Received Date: 2003-07-04
Rev Recd Date: 2003-09-24
Publish Date: 2003-12-20

Abstract

The optimized system used in RAPD reaction of Carya cathayensis must be established before analying the genetic diversity ofCarya cathayensis .It is necessary to explore the reaction conditions and system .The optimal reaction mixture and amplification procedure of RAPD in Carya cathayensis were studied .The result showed that each 20 L amplification reaction solution was consisted of 2.5mgL-1 (50 ng)template DNA , 2.0L 10 Buffer , 16.67 pmols-1Taq DNA polymerase , each 0.2mmolL-1dNTPs , 2.4 mmolL-1MgCl2 , 0.15 10-3 mmolL-1 primer .The PCR amplification program is predenature at 94 ℃for 300 s , followed by denature at 94 ℃ 30 s , annealing 38 ℃for 30 s , extension 72 ℃ for 90 s , cycling 38 times , last extension 72 ℃ 420 s . [ Ch , 5 fig .2 tab .6 ref .]
- forest tree genetics and breeding,
- Carya cathayensis,
- RAPD,
- reaction system,
- optimization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The optimal reaction system of RAPD in Carya cathayensis

1. School of Biological Science and Biotechnology , Beijing Forestry University , Beijing 10083, China;

2. School of Life Sciences, Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

3. College of Forest Resources and Environment , Nanjing Forestry University , Nanjing 210037, Jiangsu, China

Funds:

浙江省科学技术厅重大招标项目(0211025); 浙江省自然科学基金重大项目(ZA0208); 杭州市科技局重大攻关项目 (2002112A12)

Author Bio:

Corresponding author: 王正加(1972-), 男, 浙江三门人, 讲师, 从事植物生物技术研究。

Received Date: 2003-07-04

Rev Recd Date: 2003-09-24

Publish Date: 2003-12-20

Key words:

Abstract: The optimized system used in RAPD reaction of Carya cathayensis must be established before analying the genetic diversity ofCarya cathayensis .It is necessary to explore the reaction conditions and system .The optimal reaction mixture and amplification procedure of RAPD in Carya cathayensis were studied .The result showed that each 20 L amplification reaction solution was consisted of 2.5mgL-1 (50 ng)template DNA , 2.0L 10 Buffer , 16.67 pmols-1Taq DNA polymerase , each 0.2mmolL-1dNTPs , 2.4 mmolL-1MgCl2 , 0.15 10-3 mmolL-1 primer .The PCR amplification program is predenature at 94 ℃for 300 s , followed by denature at 94 ℃ 30 s , annealing 38 ℃for 30 s , extension 72 ℃ for 90 s , cycling 38 times , last extension 72 ℃ 420 s . [ Ch , 5 fig .2 tab .6 ref .]

The optimal reaction system of RAPD in Carya cathayensis

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The optimal reaction system of RAPD in Carya cathayensis

Corresponding author: 王正加(1972-), 男, 浙江三门人, 讲师, 从事植物生物技术研究。

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The optimal reaction system of RAPD in Carya cathayensis

Corresponding author: 王正加(1972-), 男, 浙江三门人, 讲师, 从事植物生物技术研究。

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