Volume 25 Issue 3
Jun.  2018
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WANG Zu-liang, DING Li-xia, ZHAO Ming-shui, CHENG Xiao-yuan, SHEN Qian. Genetic diversity of Ostrya rehderiana revealed by RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 304-308.
Citation: WANG Zu-liang, DING Li-xia, ZHAO Ming-shui, CHENG Xiao-yuan, SHEN Qian. Genetic diversity of Ostrya rehderiana revealed by RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 304-308.

Genetic diversity of Ostrya rehderiana revealed by RAPD markers

  • Received Date: 2007-11-09
  • Rev Recd Date: 2008-01-18
  • Publish Date: 2008-06-10
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Genetic diversity of Ostrya rehderiana revealed by RAPD markers

Abstract: Ostrya rehderiana is under first class national in China, only distributing in National Nature Reserve of Mount Tianmu, Zhejiang Province, and having only five wild trees(namely No.1,No.2,No.3,No.4, and No.5) in the world. The five trees were fingerprinted using random amplified polymorphic DNA(RAPD) markers with 18 primers being screened. A total of 176 DNA base pair(bp) fragments ranging from 200 bp to 2 800 bp were amplified with 88 of these bands being polymorphic(50%). Based on genetic distances, relationships among the five individuals were constructed using Unweighted Pair Group Method with Arithmetic Mean(UPGMA). Genetic distance positively correlated with geographic distance. Because No.1 was located further from the other four, it was clustered into one group alone. Therefore, more attention should be given to the tree of No.1

WANG Zu-liang, DING Li-xia, ZHAO Ming-shui, CHENG Xiao-yuan, SHEN Qian. Genetic diversity of Ostrya rehderiana revealed by RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 304-308.
Citation: WANG Zu-liang, DING Li-xia, ZHAO Ming-shui, CHENG Xiao-yuan, SHEN Qian. Genetic diversity of Ostrya rehderiana revealed by RAPD markers[J]. Journal of Zhejiang A&F University, 2008, 25(3): 304-308.

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