Adventitious bud induction with immature embryo of <EM>Carya cathayensis</EM>

WAN Jun-li; HUANG Jian-qin; XIA Guo-hua; ZHANG Qi-xiang; HUANG Li-chun

Volume 26 Issue 5

Oct. 2009

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WAN Jun-li, HUANG Jian-qin, XIA Guo-hua, ZHANG Qi-xiang, HUANG Li-chun. Adventitious bud induction with immature embryo of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2009, 26(5): 762-766.

Citation:

WAN Jun-li, HUANG Jian-qin, XIA Guo-hua, ZHANG Qi-xiang, HUANG Li-chun. Adventitious bud induction with immature embryo of Carya cathayensis[J]. Journal of Zhejiang A&F University, 2009, 26(5): 762-766.

Adventitious bud induction with immature embryo of Carya cathayensis

1.
The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College， Lin’an 311300，Zhejiang，China

More Information

Corresponding author: HUANG Jian-qin
Publish Date: 2009-10-20

Abstract

Developmental stages for explants of immature embryos of Carya cathayensis at 60，75，and 100-day after full bloom，different plant growth regulators，such as potassium，6-BA，and 2，4-D and basic media were studied. All media were supplenented with MT vitamin + 20 g·L－1 D-glucose + 10 mg·L-1 Ad + 500 mg·L-1 CH to induce adventitious-buds. Results of a starting-culture plant growth regulator for adventitious bud induction and regeneration showed that 100-day after full bloom there were better developed stages of immature embryos from induced adventitious buds with 0.01 mg·L-1 potassium + 3 mg·L-1 6-BA considered the most effective. When the concentration of potassium was constant，with an increasing concentration of 6-BA，adventitious buds gradually increased. However in a concentration of 10 mg·L-1 the buds were vitrified. Additionally，2，4-D was detrimental to adventitious buds development，and MS（Murashige and Skoog） was the best basal medium.［Ch，1 fig. 2 tab. 16 ref.］
- botany,
- Carya cathayensis,
- immature embryo,
- tissue culture,
- adventitious buds

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Adventitious bud induction with immature embryo of Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College， Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

Publish Date: 2009-10-20

Key words:

Abstract: Developmental stages for explants of immature embryos of Carya cathayensis at 60，75，and 100-day after full bloom，different plant growth regulators，such as potassium，6-BA，and 2，4-D and basic media were studied. All media were supplenented with MT vitamin + 20 g·L－1 D-glucose + 10 mg·L-1 Ad + 500 mg·L-1 CH to induce adventitious-buds. Results of a starting-culture plant growth regulator for adventitious bud induction and regeneration showed that 100-day after full bloom there were better developed stages of immature embryos from induced adventitious buds with 0.01 mg·L-1 potassium + 3 mg·L-1 6-BA considered the most effective. When the concentration of potassium was constant，with an increasing concentration of 6-BA，adventitious buds gradually increased. However in a concentration of 10 mg·L-1 the buds were vitrified. Additionally，2，4-D was detrimental to adventitious buds development，and MS（Murashige and Skoog） was the best basal medium.［Ch，1 fig. 2 tab. 16 ref.］

Adventitious bud induction with immature embryo of Carya cathayensis

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通讯作者: 陈斌, bchen63@163.com

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Adventitious bud induction with immature embryo of Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College， Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

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Adventitious bud induction with immature embryo of Carya cathayensis

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通讯作者: 陈斌, bchen63@163.com

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Adventitious bud induction with immature embryo of Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College， Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

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