Detection of <EM>Camellia oleifera</EM> root rot pathogen with nested-PCR

LI He; SONG Guang-tao; HE Mo-jun; HAO Yan; ZHOU Guo-ying

Volume 26 Issue 6

Dec. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(6): 849-853

LI He, SONG Guang-tao, HE Mo-jun, HAO Yan, ZHOU Guo-ying. Detection of Camellia oleifera root rot pathogen with nested-PCR[J]. Journal of Zhejiang A&F University, 2009, 26(6): 849-853.

Citation:

LI He, SONG Guang-tao, HE Mo-jun, HAO Yan, ZHOU Guo-ying. Detection of Camellia oleifera root rot pathogen with nested-PCR[J]. Journal of Zhejiang A&F University, 2009, 26(6): 849-853.

Detection of Camellia oleifera root rot pathogen with nested-PCR

1.
College of Forestry，Central South University of Forestry & Technology，Changsha 410004，Hunan，China

More Information

Corresponding author: ZHOU Guo-ying
Publish Date: 2009-12-20

Abstract

A species-specific polymerase chain reaction （PCR） assay for rapid and accurate detection of the pathogenic root rot of diseased Camellia oleifera plant tissues was developed. Based on differences in internal transcribed spacer（ITS） sequences of Fusarium proliferatum，a pair of species-specific primers，GF1/GR2，was designed. Other species of F. proliferatum were used to test the specificity of the primers. The GF1/GR2 primer only amplified a unique 400 bp band from the CSUFT070109 strain，and its detection sensitivity was 1 pg of genomic DNA in 25 μL reaction solution. A nested PCR procedure using ITS1/ITS4 as the first-round primers followed by GF1/GR2 increased detection sensitivity 10 000-fold to 100 ag. This assay detected the pathogen rapidly and accurately meaning methods developed here could simplify both plant disease diagnosis and pathogen monitoring as well as guide plant disease management.［Ch，4 fig. 22 ref.］
- forest protection,
- root rot of Camellia oleifera,
- Fusarium proliferatum,
- nested-PCR,
- molecular detection

Relative Article

[1]	YE Bihuan, CHEN Youwu, SHU Jinping, ZHANG Wei, ZHANG Yabo, LI Haibo, SONG Qiyan. Screening and application of reference genes for qRT-PCR in bamboo wireworm . Journal of Zhejiang A&F University, 2021, 38(3): 644-651. doi: 10.11833/j.issn.2095-0756.20200492
[2]	LIU Xuewei, WANG Zheng, LÜ Quan, ZHANG Xueping, WANG Xiuqin, KONG Xiangbo, ZHANG Xingyao. Specific and rapid detection of the pathogenic fungi associated with Ips subelongatus . Journal of Zhejiang A&F University, 2018, 35(1): 128-134. doi: 10.11833/j.issn.2095-0756.2018.01.017
[3]	LIU Yilai, WANG Jianghong, ZHANG Jing, ZHU Danni, MAO Jiajun, ZHOU Xiang. Influence of aphid-specific pathogen Conidiobolus obscurus on population dynamics of bamboo aphids and the aphid-control effect . Journal of Zhejiang A&F University, 2017, 34(5): 907-914. doi: 10.11833/j.issn.2095-0756.2017.05.018
[4]	ZHU Zhixiang, SHI Haojie, LEI Feibin, ZHANG Chuanqing. Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR . Journal of Zhejiang A&F University, 2016, 33(2): 364-368. doi: 10.11833/j.issn.2095-0756.2016.02.024
[5]	LI Hongbin, ZHU Chengqi, ZHOU Xiang, MA Liangjin, SU Xiu. Morphological and molecular identification of Heteroepichloë sasae isolated from Phyllostachys iridescens . Journal of Zhejiang A&F University, 2016, 33(6): 1040-1044. doi: 10.11833/j.issn.2095-0756.2016.06.016
[6]	WANG Xuan, MA Liangjin, LÜ Quan, MENG Xianjing, ZHANG Xingyao. Identification of the pathogens causing stem canker on Carya cathayensis . Journal of Zhejiang A&F University, 2014, 31(2): 238-245. doi: 10.11833/j.issn.2095-0756.2014.02.012
[7]	ZHANG Xin, SHEN Aihua, LIN Yongjing, WANG Qiuqin, LIN Haiping. Infection and nodulation with Casuarina equisetifolia-Frankia symbiosis . Journal of Zhejiang A&F University, 2013, 30(5): 669-673. doi: 10.11833/j.issn.2095-0756.2013.05.006
[8]	HUANG Yiming, YANG Shuzhen, ZHAO Mingshui, CHEN Jiaqi, WU Jianqi, WEN Guosheng. Cryptomeria fortunei tumor disease and a removal method . Journal of Zhejiang A&F University, 2013, 30(6): 910-913. doi: 10.11833/j.issn.2095-0756.2013.06.016
[9]	FU Jianguo, LIU Jinliang, YANG Xiaojun, AN Yulin, LUO Jiayan. DNA extraction and molecular identification of imported Dalbergia wood . Journal of Zhejiang A&F University, 2013, 30(4): 627-632. doi: 10.11833/j.issn.2095-0756.2013.04.025
[10]	GONG Xiao-feng, ZHU Yun-feng, YANG Shu-zhen, JIANG Wen-wei, LUO Jia-qi. Pathogenisis regularity of the gall diseases of Cryptomeria fortunei in Mount Tianmu . Journal of Zhejiang A&F University, 2012, 29(6): 904-909. doi: 10.11833/j.issn.2095-0756.2012.06.015
[11]	HUA Zheng-yuan, WANG Jing-tian, LIU Jian, WANG Hao-jie, SHU Jin-ping, XU Tian-sen. Insect pests of Camellia oleifera and their natural enemies in Quzhou，China . Journal of Zhejiang A&F University, 2012, 29(2): 232-243. doi: 10.11833/j.issn.2095-0756.2012.02.013
[12]	TIAN Tian, SHEN Zhen-ming, XU Qiu-fang, LIU Bo-rong. Screening and identification of inhibiting strains for Carya cathayensis canker disease . Journal of Zhejiang A&F University, 2012, 29(1): 58-64. doi: 10.11833/j.issn.2095-0756.2012.01.011
[13]	YU Hai-ying, CHAI Shou-quan, ZHOU Yu-jiang, ZHAO Jun. Relationship between Valsa sordida and abnormal coldness in spring . Journal of Zhejiang A&F University, 2011, 28(6): 927-930. doi: 10.11833/j.issn.2095-0756.2011.06.015
[14]	YANG Yong-gang, WU Xiao-qin. Research progress on pathogens of witches’ broom of bamboo . Journal of Zhejiang A&F University, 2011, 28(1): 144-148. doi: 10.11833/j.issn.2095-0756.2011.01.023
[15]	GONG Xiao-feng, ZHU Yun-feng, LIU Liang, YANG Shu-zhen, GE Xiao-feng, GAN Qi-xing, YE Jian-ren, JIANG Ping. Causal agent of Cryptomeria fortunei gall disease . Journal of Zhejiang A&F University, 2011, 28(2): 339-342. doi: 10.11833/j.issn.2095-0756.2011.02.027
[16]	MA Liang-jin, WU Mei-qing, SU Xiu, CHEN An-liang, ZHANG Li-qin. Antifungal activity in vitro with extracts from the husk of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(5): 620-624.
[17]	LIN Jun-yang, MA Liang-jin, CHEN An-liang, ZHANG Li-qin. Chemical components and antifungal activities of extracts from the husk of Carya cathayensis . Journal of Zhejiang A&F University, 2009, 26(1): 100-104.
[18]	HI Dong-hui, LIU Hong-bo, YANG Xiao-feng, ZHANG Yu, CHEN An-liang, ZHANG Li-qin. Antifungal activity of the essential oil from Magnolia cylindrica on nine phytopathogenic fungi . Journal of Zhejiang A&F University, 2009, 26(2): 223-227.
[19]	SU Xiu, MA Liang-jin, CHEN An-liang, ZHANG Li-qin. Antifungal and antibacterial activity of extracts from the husk of Carya cathayensis . Journal of Zhejiang A&F University, 2008, 25(3): 355-358.
[20]	YANG Yan-hong, CHEN Yu-hui, ZHU Yun-feng. Isolation and identification of Cronartium ribicola mycoparasites in southwest China . Journal of Zhejiang A&F University, 2005, 22(4): 414-419.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3608) PDF downloads(150) Cited by()

Proportional views

Detection of Camellia oleifera root rot pathogen with nested-PCR

1. College of Forestry，Central South University of Forestry & Technology，Changsha 410004，Hunan，China

Corresponding author: ZHOU Guo-ying

Publish Date: 2009-12-20

Key words:

Abstract: A species-specific polymerase chain reaction （PCR） assay for rapid and accurate detection of the pathogenic root rot of diseased Camellia oleifera plant tissues was developed. Based on differences in internal transcribed spacer（ITS） sequences of Fusarium proliferatum，a pair of species-specific primers，GF1/GR2，was designed. Other species of F. proliferatum were used to test the specificity of the primers. The GF1/GR2 primer only amplified a unique 400 bp band from the CSUFT070109 strain，and its detection sensitivity was 1 pg of genomic DNA in 25 μL reaction solution. A nested PCR procedure using ITS1/ITS4 as the first-round primers followed by GF1/GR2 increased detection sensitivity 10 000-fold to 100 ag. This assay detected the pathogen rapidly and accurately meaning methods developed here could simplify both plant disease diagnosis and pathogen monitoring as well as guide plant disease management.［Ch，4 fig. 22 ref.］

Detection of Camellia oleifera root rot pathogen with nested-PCR

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Detection of Camellia oleifera root rot pathogen with nested-PCR

1. College of Forestry，Central South University of Forestry & Technology，Changsha 410004，Hunan，China

Corresponding author: ZHOU Guo-ying

HTML

Catalog

Detection of Camellia oleifera root rot pathogen with nested-PCR

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Detection of Camellia oleifera root rot pathogen with nested-PCR

1. College of Forestry，Central South University of Forestry & Technology，Changsha 410004，Hunan，China

Corresponding author: ZHOU Guo-ying

HTML

Catalog

Export File

Citation

Format

Content