Cloning and SNP analysis of <em>PPO</em> genes with cultivars of <em>Phyllostachys nigra</em>

XIAO Guohui; WANG Yi; LOU Yongfeng; LIN Xinchun; GUO Xiaoqin; FANG Wei

doi:10.11833/j.issn.2095-0756.2013.04.008

Volume 30 Issue 4

Jul. 2013

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XIAO Guohui, WANG Yi, LOU Yongfeng, LIN Xinchun, GUO Xiaoqin, FANG Wei. Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra[J]. Journal of Zhejiang A&F University, 2013, 30(4): 511-516. doi: 10.11833/j.issn.2095-0756.2013.04.008

Citation:

XIAO Guohui, WANG Yi, LOU Yongfeng, LIN Xinchun, GUO Xiaoqin, FANG Wei. Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra[J]. Journal of Zhejiang A&F University, 2013, 30(4): 511-516. doi: 10.11833/j.issn.2095-0756.2013.04.008

Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2013.04.008

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: GUO Xiaoqin
Received Date: 2012-08-02
Rev Recd Date: 2012-10-17
Publish Date: 2013-08-20

Abstract

Polyphenol oxidase（PPO） fragments were cloned from cultivars of Phyllostachys nigra using a pair of primers designed according to a conserved region（CuA and CuB） of a PPO gene sequence in Poaceae family and analyzed with a sequence analysis. The genetic relationship of cultivars was also analyzed based on these fragments using DNAMAN software. Results of the sequence analysis showed that identity was 92.35% in nucleotide acid and 81.83% in amino acid. A truncated protein of the gene was found in some cultivars of P. nigra，such asOne-year-purpleandPurple Groove. Single?鄄nucleotide polymorphism（SNP） sites were also found among these sequences. This could lead to non-synonymous mutations.［Ch，2 fig. 3 tab. 20 ref.］
- botany,
- Phyllostachys nigra,
- polyphenol oxidase（PPO）gene,
- single-nucleotide polymorphism （SNP）,
- genetic relationship

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: GUO Xiaoqin

Received Date: 2012-08-02

Rev Recd Date: 2012-10-17

Publish Date: 2013-08-20

Key words:

Abstract: Polyphenol oxidase（PPO） fragments were cloned from cultivars of Phyllostachys nigra using a pair of primers designed according to a conserved region（CuA and CuB） of a PPO gene sequence in Poaceae family and analyzed with a sequence analysis. The genetic relationship of cultivars was also analyzed based on these fragments using DNAMAN software. Results of the sequence analysis showed that identity was 92.35% in nucleotide acid and 81.83% in amino acid. A truncated protein of the gene was found in some cultivars of P. nigra，such asOne-year-purpleandPurple Groove. Single?鄄nucleotide polymorphism（SNP） sites were also found among these sequences. This could lead to non-synonymous mutations.［Ch，2 fig. 3 tab. 20 ref.］

Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2013.04.008

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Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2013.04.008

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: GUO Xiaoqin

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Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2013.04.008

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Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

doi: 10.11833/j.issn.2095-0756.2013.04.008

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: GUO Xiaoqin

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