Identification of <i>S</i>-genotypes from five cultivars of <i>Pyrus pyrifolia</i>

ZENG Yan-ling; TAN Xiao-feng; ZHANG Dang-quan; ZENG Xiao-feng; LI Xiu-gen; LIU Xian-xiong

Dec. 2007

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(6): 654-660

ZENG Yan-ling, TAN Xiao-feng, ZHANG Dang-quan, ZENG Xiao-feng, LI Xiu-gen, LIU Xian-xiong. Identification of S-genotypes from five cultivars of Pyrus pyrifolia[J]. Journal of Zhejiang A&F University, 2007, 24(6): 654-660.

Citation:

ZENG Yan-ling, TAN Xiao-feng, ZHANG Dang-quan, ZENG Xiao-feng, LI Xiu-gen, LIU Xian-xiong. Identification of S-genotypes from five cultivars of Pyrus pyrifolia[J]. Journal of Zhejiang A&F University, 2007, 24(6): 654-660.

Identification of S-genotypes from five cultivars of Pyrus pyrifolia

1. The Key Laboratory of Non-wood Forest Product of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;
2. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, Henan, China;
3. Lanshan Gold Field Exploitation Limited Company, Lanshan 425800, Hunan, China

Received Date: 2006-12-07
Rev Recd Date: 2007-08-20

Abstract

With natural pollination, fruit from trees having pistil self-incompatible S-alleles will degrade pollen tube RNA resulting in low yield and poor fruit quality. This research identified S-allele genotypes of pear (Pyrus) that served as an important scientific foundation for determining cultivars for pollination and crossbreeding, as well as for establishing a basis on inheriting desired characteristics and improving pear cultivars. Five kinds of pear genomic DNA material were tested based on primary structural characteristics of S-alleles. After designing and synthesizing specific primers and using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) system along with TA clone technique, results of sequences were analyzed with bioinformatics software (GeneDoc). Two S-alleles of amplified segments, which had similar sizes, were isolated and identified with their S-genotypes identified as cultivars of Pyrus pyrifolia, namely ‘Jingyu’, S₄S₂₄; ‘Jinshui’No.2, S₃S₂₁; ‘Xinya’, S₄S₂₄; ‘Xizilv', S₄S₁₃; and ‘Qingkui’, S₁S₁₃.
- horticulture,
- Pyrus pyrifolia (pear),
- S-genotype,
- isolate and identify

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References


[2]	TERAMI H,TORIKATA H,SHIMAZU Y. Analysis of the sterility-factors existing in varieties of the Japanese pear (Pyrus serotina Rehd. var. culta Rehd.)(In Japanese,English abstract)[J].Stud Hort Inst Kyoto Imp Univ,1946,3:267-271.
[3]	ISHIMIZU T,SHINKAWA T,SAKIYAMA F,et al. Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility[J].Plant Mol Biol,1998,37:931-941.
[4]	HIRATSUKA S,OKADA Y,KAWAI Y,et al. Expression and inheritances of S-proteins in self-compatible and-incompatible Japanese pears[J]. J Jpn Soc Hort Sci,1995,64:479-484.
[5]	ISHIMIZU T,SATO Y,SAITO T,et al. Identification and partial amino-acid sequences of seven S-RNases associated with self-incompatibility of the Japanese pear,Pyrus pyrifolia Nakai[J].J Biochem,1996,120:326-334.
[6]	TAKASAKI1 T,OKADA K,CASTILLO C,et al. Sequence of the S₉-RNase cDNA and PCR-RFLP system for discriminating S₁-to S₉-allele in Japanese pear[J].Euphytica,2004,135:157-167.
[7]	CASTILLO C,TAKASAK T,SATTO T,et al. Cloning of the S₈-RNase (S₈-allele) of Japanese pear (Pyrus pyrifolia Nakai)[J].Plant Biotechnol J,2002,19:1-6.

[9]	KIM H,HIRATA Y,NOU I S. Determination of S-Genotypes of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR-RFLP analyses[J]. Mol Cell,2002,13:444-451.






[16]	TAN X F,ZHANG L,WUYUN T N,et al. Molecular identification of two new self-incompatible alleles (S-alleles) in Chinese pear (Pyrus bretschneideri)[J]. J Plant Physiol Mol Biol,2007,33(1):61-70.





[22]	MATSUURA T,SAKAI H,UNNO M,et al. Crystal structure at 1.5-Åresolution of Pyrus pyrifolia pistil ribonuclease responsible for gametophytic self-incompatibility[J]. J Biol Chem,2001,276(48):45261-45269.
[23]	ISHIMIZU T,INOUE K,SHIMONAKA M,et al. PCR-based method for identifying the S-genotypes of Japanese pear cultvars[J]. Theor Appl Genet,1999,98:961-967.
[24]	USHIJIMA K,SASSA H,TAO R,et al. cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis):primary structural features and sequence diversity of the S-RNases in Rosaceae[J]. Mol Gen Genet,1998,260:261-268.
[25]	USHIJIMA K,SASSA H,HIRANO H. Characterization of the flanking regions of the S-RNase genes of Japanese pear (Pyrus serotina) and apple (Malus×domestica)[J].Gene,1998,211:159-167.
[26]	TAMURA M,USHIJIMA K,SASSA H,et al. Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis[J]. Theor Appl Genet,2000,101:344-349.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Identification of S-genotypes from five cultivars of Pyrus pyrifolia

1. The Key Laboratory of Non-wood Forest Product of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;

2. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, Henan, China;

3. Lanshan Gold Field Exploitation Limited Company, Lanshan 425800, Hunan, China

Received Date: 2006-12-07

Rev Recd Date: 2007-08-20

Key words:

Abstract: With natural pollination, fruit from trees having pistil self-incompatible S-alleles will degrade pollen tube RNA resulting in low yield and poor fruit quality. This research identified S-allele genotypes of pear (Pyrus) that served as an important scientific foundation for determining cultivars for pollination and crossbreeding, as well as for establishing a basis on inheriting desired characteristics and improving pear cultivars. Five kinds of pear genomic DNA material were tested based on primary structural characteristics of S-alleles. After designing and synthesizing specific primers and using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) system along with TA clone technique, results of sequences were analyzed with bioinformatics software (GeneDoc). Two S-alleles of amplified segments, which had similar sizes, were isolated and identified with their S-genotypes identified as cultivars of Pyrus pyrifolia, namely ‘Jingyu’, S₄S₂₄; ‘Jinshui’No.2, S₃S₂₁; ‘Xinya’, S₄S₂₄; ‘Xizilv', S₄S₁₃; and ‘Qingkui’, S₁S₁₃.

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[2]	TERAMI H,TORIKATA H,SHIMAZU Y. Analysis of the sterility-factors existing in varieties of the Japanese pear (Pyrus serotina Rehd. var. culta Rehd.)(In Japanese,English abstract)[J].Stud Hort Inst Kyoto Imp Univ,1946,3:267-271.
[3]	ISHIMIZU T,SHINKAWA T,SAKIYAMA F,et al. Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility[J].Plant Mol Biol,1998,37:931-941.
[4]	HIRATSUKA S,OKADA Y,KAWAI Y,et al. Expression and inheritances of S-proteins in self-compatible and-incompatible Japanese pears[J]. J Jpn Soc Hort Sci,1995,64:479-484.
[5]	ISHIMIZU T,SATO Y,SAITO T,et al. Identification and partial amino-acid sequences of seven S-RNases associated with self-incompatibility of the Japanese pear,Pyrus pyrifolia Nakai[J].J Biochem,1996,120:326-334.
[6]	TAKASAKI1 T,OKADA K,CASTILLO C,et al. Sequence of the S₉-RNase cDNA and PCR-RFLP system for discriminating S₁-to S₉-allele in Japanese pear[J].Euphytica,2004,135:157-167.
[7]	CASTILLO C,TAKASAK T,SATTO T,et al. Cloning of the S₈-RNase (S₈-allele) of Japanese pear (Pyrus pyrifolia Nakai)[J].Plant Biotechnol J,2002,19:1-6.
[8]	谭晓风,乌云塔娜,中西ラツ,等.中国砂梨7个自交不亲和新基因的分离与测序[J].中南林学院学报,2005,25(4):1-6.
[9]	KIM H,HIRATA Y,NOU I S. Determination of S-Genotypes of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR-RFLP analyses[J]. Mol Cell,2002,13:444-451.
[10]	谭晓风,乌云塔娜,中西ラツ,等.中国梨品种自交不亲和新基因的分离鉴定[J].中南林学院学报,2005,25(1):1-3.
[11]	乌云塔娜,谭晓风.中国白梨7个主栽品种S基因型的确定及2个新S-RNase基因的鉴定[J].中南林学院学报,2005,25(4):7-12.
[12]	谭晓风,毕方铖,乌云塔娜.中国白梨9个主栽品种S基因型的确定及S₂₉-RNase新基因的分离鉴定[J].中南林学院学报,2005,25(4):13-16.
[13]	乌云塔娜,谭晓风,李秀根,等.梨自交不亲和新基因S₁₂-RNase的分离鉴定及序列分析[J].林业科学,2006,42(4):117-121.
[14]	乌云塔娜,谭晓风,曹玉芬,等.白梨新S基因的克隆[J].园艺学报,2006,33(2):385-388.
[15]	张妤艳,吴俊,衡伟,等.京白梨等品种S基因型鉴定及新基因S₂₈和S₃₀的核苷酸序列分析[J].园艺学报,2006,33(3):496-500.
[16]	TAN X F,ZHANG L,WUYUN T N,et al. Molecular identification of two new self-incompatible alleles (S-alleles) in Chinese pear (Pyrus bretschneideri)[J]. J Plant Physiol Mol Biol,2007,33(1):61-70.
[17]	谭晓风,曾艳玲,乌云塔娜,等.雪花梨及其亲缘品种S基因型的确定[J].果树学报,2006,23(3):355-358.
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[20]	谭晓风,漆龙霖,黄晓光,等.山茶属植物叶片DNA抽提[J].中南林学院学报,1999,19(4):16-21.
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[22]	MATSUURA T,SAKAI H,UNNO M,et al. Crystal structure at 1.5-Åresolution of Pyrus pyrifolia pistil ribonuclease responsible for gametophytic self-incompatibility[J]. J Biol Chem,2001,276(48):45261-45269.
[23]	ISHIMIZU T,INOUE K,SHIMONAKA M,et al. PCR-based method for identifying the S-genotypes of Japanese pear cultvars[J]. Theor Appl Genet,1999,98:961-967.
[24]	USHIJIMA K,SASSA H,TAO R,et al. cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis):primary structural features and sequence diversity of the S-RNases in Rosaceae[J]. Mol Gen Genet,1998,260:261-268.
[25]	USHIJIMA K,SASSA H,HIRANO H. Characterization of the flanking regions of the S-RNase genes of Japanese pear (Pyrus serotina) and apple (Malus×domestica)[J].Gene,1998,211:159-167.
[26]	TAMURA M,USHIJIMA K,SASSA H,et al. Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis[J]. Theor Appl Genet,2000,101:344-349.
[27]	方瑾.植物的生殖讲座(五):被子植物的自交不亲和性[J].生物学通报,1996,30(1):28-30.

Identification of S-genotypes from five cultivars of Pyrus pyrifolia

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