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毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式

王绍良 张雯宇 高志民 周明兵 杨克彬 宋新章

王绍良, 张雯宇, 高志民, 周明兵, 杨克彬, 宋新章. 毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210471
引用本文: 王绍良, 张雯宇, 高志民, 周明兵, 杨克彬, 宋新章. 毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210471
WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210471
Citation: WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210471

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毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式

doi: 10.11833/j.issn.2095-0756.20210471
基金项目: 国家自然科学基金资助项目(31930075,32125027)
详细信息
    作者简介: 王绍良(ORCID: 0000-0002-2342-7723),从事植物生态学研究。E-mail: 2248837957@qq.com
    通信作者: 宋新章(ORCID: 0000-0003-2434-7466),教授,博士,博士生导师,从事人工林生产力与碳氮磷生物地球化学循环研究。E-mail: xzsong@126.com
  • 中图分类号: Q943.2;TS721.+2

Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis

  • 摘要:   目的  鉴定毛竹Phyllostachys edulis磷转运蛋白Ⅰ (phosphate transporter 1, PHTⅠ)家族基因,分析其表达模式。  方法  利用生物信息学方法,鉴定毛竹PHTⅠ家族成员,分析基因启动子调控元件、编码蛋白的理化性质、基因结构、氨基酸保守基序、基因在染色体的位置、组织表达特异性、基因适应性进化及系统进化等。  结果  毛竹中共鉴定出20个PHTⅠ家族基因 (PePHTs),分布在10条染色体上,均定位于细胞膜。每个基因都含有1~2个内含子,PePHTs启动子序列中包含干旱、低温等非生物胁迫以及赤霉素等激素类响应元件。毛竹PHTⅠ大部分为碱性蛋白质,分子量为48.61~71.89 kDa,理论等电点为6.84~9.30,疏水性值均大于0,都属于疏水蛋白。基因适应性进化分析显示:大多数PePHTs的选择压力值小于0,说明多数基因受到负选择压力。转录组表达图谱表明:PePHTs在不同组织中的表达存在差异性,说明该基因家族在毛竹生长发育过程中发挥着不同的作用。系统进化树表明:PePHTs都聚类在第Ⅰ亚家族,并且优先和水稻Oryza sativa聚类在同一支上。  结论  PHTⅠ家族在植物吸收和转运磷的过程中扮演了重要作用。本研究结果为深入研究毛竹PHTⅠ家族基因的功能奠定了理论基础。图5表1参45
  • 图  1  毛竹PHTⅠ家族基因的结构

    Figure  1  Structures analysis of PHTⅠ gene family in Ph. edulis

    图  2  毛竹PHTⅠ家族基因启动子区顺式作用元件位置信息

    Figure  2  Location information of cis-acting regulatory elements identified in the promoter region of PHTⅠ gene family in Ph. edulis

    图  3  毛竹、拟南芥和水稻PHT家族基因系统进化树

    Figure  3  Phylogentic tree of PHT gene family from Ph. edulis, Arabidopsis thaliana and Oryza sative

    图  4  毛竹PHTⅠ基因在染色体上的位置

    Figure  4  Chromosomal location of PHTⅠ genes from Ph. edulis

    图  5  PePHTs在毛竹6个部位的表达

    Figure  5  Expression analysis of PePHTs in 6 parts of Ph. edulis

    表  1  毛竹PHTⅠ家族基因编码蛋白序列的理化性质

    Table  1.   Physicochemical properties of proteins encoded by PHTⅠ gene family in Phyllostachy edulis

    基因名称基因登录号氨基酸数/个理论等电点分子量/kDa疏水性值脂肪族氨基酸指数亚细胞定位
    PePHT1 PH02Gene03602 696 7.63 76.37 0.269 92.56 细胞膜
    PePHT2 PH02Gene11006 531 9.10 57.69 0.538 98.91 细胞膜
    PePHT3 PH02Gene14509 541 9.15 59.64 0.322 95.10 细胞膜
    PePHT4 PH02Gene21291 532 8.45 58.05 0.371 89.40 细胞膜
    PePHT5 PH02Gene24702 536 8.71 59.02 0.321 90.80 细胞膜
    PePHT6 PH02Gene37931 547 8.31 58.97 0.412 92.32 细胞膜
    PePHT7 PH02Gene39948 558 8.85 60.96 0.313 93.58 细胞膜
    PePHT8 PH02Gene44007 536 8.03 58.49 0.408 91.27 细胞膜
    PePHT9 PH02Gene44009 574 8.60 62.35 0.449 94.79 细胞膜
    PePHT10 PH02Gene48053 507 8.01 55.77 0.259 87.59 细胞膜
    PePHT11 PH02Gene48969 598 9.21 59.84 0.204 83.79 细胞膜
    PePHT12 PH02Gene49859 567 8.99 61.45 0.376 92.31 细胞膜
    PePHT13 PH02Gene50239 554 8.95 60.55 0.329 93.92 细胞膜
    PePHT14 PH02Gene21248 518 8.02 57.42 0.494 105.04 细胞膜
    PePHT15 PH02Gene21249 655 6.84 71.89 0.226 90.87 细胞膜
    PePHT16 PH02Gene21250 505 9.30 55.46 0.473 105.07 细胞膜
    PePHT17 PH02Gene21252 432 9.24 48.61 0.348 101.83 细胞膜
    PePHT18 PH02Gene47590 520 8.67 58.31 0.324 90.25 细胞膜
    PePHT19 PH02Gene47591 563 8.92 62.63 0.314 94.81 细胞膜
    PePHT20 PH02Gene49564 527 8.04 58.50 0.391 97.53 细胞膜
    下载: 导出CSV
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  • 文章访问数:  85
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出版历程
  • 收稿日期:  2021-07-05
  • 录用日期:  2021-12-10
  • 修回日期:  2021-12-06

毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式

doi: 10.11833/j.issn.2095-0756.20210471
    基金项目:  国家自然科学基金资助项目(31930075,32125027)
    作者简介:

    王绍良(ORCID: 0000-0002-2342-7723),从事植物生态学研究。E-mail: 2248837957@qq.com

    通信作者: 宋新章(ORCID: 0000-0003-2434-7466),教授,博士,博士生导师,从事人工林生产力与碳氮磷生物地球化学循环研究。E-mail: xzsong@126.com
  • 中图分类号: Q943.2;TS721.+2

摘要:   目的  鉴定毛竹Phyllostachys edulis磷转运蛋白Ⅰ (phosphate transporter 1, PHTⅠ)家族基因,分析其表达模式。  方法  利用生物信息学方法,鉴定毛竹PHTⅠ家族成员,分析基因启动子调控元件、编码蛋白的理化性质、基因结构、氨基酸保守基序、基因在染色体的位置、组织表达特异性、基因适应性进化及系统进化等。  结果  毛竹中共鉴定出20个PHTⅠ家族基因 (PePHTs),分布在10条染色体上,均定位于细胞膜。每个基因都含有1~2个内含子,PePHTs启动子序列中包含干旱、低温等非生物胁迫以及赤霉素等激素类响应元件。毛竹PHTⅠ大部分为碱性蛋白质,分子量为48.61~71.89 kDa,理论等电点为6.84~9.30,疏水性值均大于0,都属于疏水蛋白。基因适应性进化分析显示:大多数PePHTs的选择压力值小于0,说明多数基因受到负选择压力。转录组表达图谱表明:PePHTs在不同组织中的表达存在差异性,说明该基因家族在毛竹生长发育过程中发挥着不同的作用。系统进化树表明:PePHTs都聚类在第Ⅰ亚家族,并且优先和水稻Oryza sativa聚类在同一支上。  结论  PHTⅠ家族在植物吸收和转运磷的过程中扮演了重要作用。本研究结果为深入研究毛竹PHTⅠ家族基因的功能奠定了理论基础。图5表1参45

English Abstract

王绍良, 张雯宇, 高志民, 周明兵, 杨克彬, 宋新章. 毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210471
引用本文: 王绍良, 张雯宇, 高志民, 周明兵, 杨克彬, 宋新章. 毛竹磷转运蛋白Ⅰ家族基因鉴定及表达模式[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210471
WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210471
Citation: WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210471

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