Identification of S-genotypes from five cultivars of Pyrus pyrifolia
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摘要: 果树雌蕊自交不亲和基因（S基因）产物能降解具有相同S基因型的花粉管核糖核酸，导致自然授粉结实率低，果实品质差。确定不同梨品种S基因型，为配置授粉树和杂交育种提供重要科学依据，并为遗传改良梨品种奠定基础。实验以5个砂梨Pyrus pyrifolia品种基因组脱氧核糖核酸为实验材料，根据S基因一级结构特征，设计合成特异引物，采用PCR-RFLP系统检测和TA克隆测序等方法，分离鉴定了这些品种扩增片段大小差异很小的2条S基因，确定了它们的S基因型，分别为晶玉Pyrus pyrifolia ‘Jingyu‘ S4S24，金水2号P.pyrifolia ‘Jinshui No.2’ S3S21，新雅P.pyrifolia ‘Xinya’ S4S24，西子绿P.pyrifolia ‘Xizilv’ S4S13，青魁P.pyrifolia ‘Qingkui’ S1S13，并且对各品种测序结果进行了初步的生物信息学分析。Abstract: With natural pollination, fruit from trees having pistil self-incompatible S-alleles will degrade pollen tube RNA resulting in low yield and poor fruit quality. This research identified S-allele genotypes of pear (Pyrus) that served as an important scientific foundation for determining cultivars for pollination and crossbreeding, as well as for establishing a basis on inheriting desired characteristics and improving pear cultivars. Five kinds of pear genomic DNA material were tested based on primary structural characteristics of S-alleles. After designing and synthesizing specific primers and using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) system along with TA clone technique, results of sequences were analyzed with bioinformatics software (GeneDoc). Two S-alleles of amplified segments, which had similar sizes, were isolated and identified with their S-genotypes identified as cultivars of Pyrus pyrifolia, namely ‘Jingyu’, S4S24; ‘Jinshui’No.2, S3S21; ‘Xinya’, S4S24; ‘Xizilv', S4S13; and ‘Qingkui’, S1S13.
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Key words:
- horticulture /
- Pyrus pyrifolia (pear) /
- S-genotype /
- isolate and identify
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