A study of effect of carbon source on properties of cellulose system during liquid fermentation process from Trichoderma reesei

Key words: 
• biochemistry /
• Trichoderma reesei /
• cellulase system /
• characteristics of enzyme production /
• balance

Abstract: In the condition of liquid fermentation , we have studied the effects of different factors on highest enzyme activity (ahe), enzyme activity ratio (rea), formation time of highest enzyme activity (FTH)and secretory rule of components in cellulase system inTrichoderma reesei with cellulase , wheat bran and a mixture of two as different carbon sources and additions of CaCO3 and yeast extract paste .The results show that there are great differences among ahe , rea and FTH of different compositions when cellulase and wheat bran are used as carbon sources .The mixed carbon source (1.0 % cellulase +2.0 %wheat bran)results in peak ahe (C1 , Cx and BG reach 76.18 10-5 , 313.25 10-5 , 135.60 10-5mols-1 and rhe (C1 , Cx and BG reach 72.18 10-5 , 331.57 10-5 , 124.29 10-5mols-1).The addition of CaCO3 can increase the ahe (C1 and Cx reach 196.21 10-5 , 254.90 10-5mols-1)and rea of C1 (100.17 10-5)and Cx (215.61 10-5mols-1) and reduce the rea of BG (57.61 10-5 mols-1), formation time of ahe of C1 and Cx distinctly .The addition of yeast extract paste can increase ahe of BG (260.61 10-5 mols-1)and reduce the rea of BG (38.9 10-5 mols-1), secretory rule dramatically .The equilibrium of all components are very different and their effects on BG are lagged behind .[ Ch , 3 fig .3 tab .12 ref .]