留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

西红花真菌性病害与生防菌鉴定研究进展

杜雪 李秀娟 桂思琦 开国银 邵果园 周伟

杜雪, 李秀娟, 桂思琦, 开国银, 邵果园, 周伟. 西红花真菌性病害与生防菌鉴定研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200809
引用本文: 杜雪, 李秀娟, 桂思琦, 开国银, 邵果园, 周伟. 西红花真菌性病害与生防菌鉴定研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200809
DU Xue, LI Xiujuan, GUI Siqi, KAI Guoyin, SHAO Guoyuan, ZHOU Wei. Research progress of fungal diseases in Crocus sativus and identification of biocontrol bacteria[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200809
Citation: DU Xue, LI Xiujuan, GUI Siqi, KAI Guoyin, SHAO Guoyuan, ZHOU Wei. Research progress of fungal diseases in Crocus sativus and identification of biocontrol bacteria[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200809

本文已在中国知网网络首发,可在知网搜索、下载并阅读全文。

西红花真菌性病害与生防菌鉴定研究进展

doi: 10.11833/j.issn.2095-0756.20200809
基金项目: 浙江省自然科学基金面上项目(LY20H280008);全国大学生创新创业训练计划(202010344024)
详细信息
    作者简介: 杜雪(ORCID: 0000-0002-8056-7319),从事植物组织培养、致病菌的分离与鉴定研究。E-mail: 1365609131@qq.com
    通信作者: 邵果园(ORCID: 0000-0001-5766-5255),副教授,从事园艺植物新品种选育与营养功效分析研究。E-mail: 360900227@qq.com。周伟(ORCID: 0000-0002-9309-5832),副教授,博士,从事药用植物种质资源创新利用、药效物质代谢合成分子调控和基因组学研究。E-mail: zhouwei19810501@163.com
  • 中图分类号: R282.2

Research progress of fungal diseases in Crocus sativus and identification of biocontrol bacteria

  • 摘要: 西红花Crocus sativus为多年生草本植物,具有活血化瘀、凉血解毒、抗癌、抗氧化等多种药用价值。由于无性繁殖及连作障碍,西红花病害日益严重;以真菌性引起的西红花球茎腐烂病害最为常见,造成西红花产量锐减,严重影响产业发展。解决真菌性病害是提高西红花产量与品质的有效途径之一。围绕西红花土壤真菌性病害、西红花内生真菌、西红花真菌病害生防菌的分离与鉴定研究进展等展开综述,分析西红花研究前景。后续研究可以从3个方向展开,一是基于现代宏基因组测序技术挖掘西红花球茎腐烂致病菌和拮抗菌,二是基于合成生物学和发酵工程技术方法实现西红花药效物质的异源高效生物合成,三是创制西红花脱毒新种质,为生产提供可靠的优质种源。本研究能为西红花真菌性病害防治、生物菌肥的开发利用等研究提供参考。表3参89
  • 表  1  西红花球茎腐烂致病真菌及其来源

    Table  1.   Summary of pathomycete isolation from rotting bulbs of C. sativus

    菌株类型分离部位参考文献菌株类型分离部位参考文献
    尖孢镰刀菌Fusarium oxysporum腐烂球茎[15]炭疽菌Anthracnose sp.腐烂球茎[34]
    腐皮镰刀菌F. solani腐烂球茎[15]红棕孔韧革菌Porostereum sp.健康球茎[35]
    巴西曲霉Aspergillus brasiliensis腐烂球茎[32]离生青霉菌P. solitum腐烂球茎[36]
    桔青霉菌Penicillium citrinum腐烂球茎[32]齐整小核菌Sclerotium rolfsii腐烂球茎[38]
    链格孢菌Alernaria alternata健康球茎[33]
    下载: 导出CSV

    表  2  已报道的西红花生防菌

    Table  2.   Biocontrol bacterium of C. sativus had been reported

    菌株名称菌株类型来源作 用参考文献
    蜂房类芽孢杆菌Paenibacillus alvei ZJUB2011-1细菌根际土壤防治球茎腐烂病     [63]
    解淀粉芽孢杆菌Bacillus amyloliquefaciens C612细菌根际土壤抑制病原菌生长     [15]
    苏云金芽孢杆菌B. thuringiensis DC1细菌根际土壤抑制病原菌,促进植株生长[65]
    巨大芽孢杆菌B. megaterium VC3细菌根际土壤抑制病原菌,促进植株生长[65]
    解淀粉芽孢杆菌B. amyloliquefaciens DC8细菌根际土壤抑制病原菌,促进植株生长[65]
    枯草芽孢杆菌B. subtilis细菌生防药剂防治球茎腐烂病     [66]
    荧光假单胞菌Pseudomonas fluorescens细菌生防药剂防治球茎腐烂病     [66]
    棘孢木霉菌Trichoderma asperellum真菌生防药剂防治球茎腐烂病     [66]
    解淀粉芽孢杆菌B. amyloliquefaciens W2细菌根际土壤防治球茎腐烂病     [67]
    下载: 导出CSV

    表  3  生防菌在其他药用植物的挖掘与验证

    Table  3.   The excavation and verification of biocontrol bacteria in other Traditional Chinese medicine plants

    菌株名称菌株类型来源功能参考文献
    顶孢属真菌Acremonium sp.真菌 健康生姜  产生胶霉毒素抑制病原菌[68]
    紫色色杆菌Chromobacterium sp. JH7细菌 人参根际土壤产生几丁质酶、蛋白酶抑制病原菌[69]
    深海链霉菌Streptomyces scopuliridis细菌 人参根际土壤产生几丁质酶、蛋白酶抑制病原菌[69]
    灰锈赤链霉菌S. griseorubiginosus放线菌川芎根茎  抑制4种川芎根腐病原菌[72]
    团孢链霉菌S. agglomeratus放线菌川芎根茎  抑制4种川芎根腐病原菌[72]
    解淀粉芽孢杆菌B. amyloliquefaciens C10细菌 人参根际土壤改变真菌群落结构[73]
    萎缩芽孢杆菌B. atrophaeus SXKF16-1细菌 黄芪根际土壤定植于根际土壤,改善土壤微生态环境[74]
    哈茨根霉Trichoderma harzianum TharDOB-31真菌 健康姜黄根茎定植于根茎,产生抗真菌化合物[75]
    下载: 导出CSV
  • [1] RAHAIEE S, MOINI S, HASHEMI M, et al. Evaluation of antioxidant activities of bioactive compounds and various extracts obtained from saffron (Crocus sativus L.): a review [J]. J Food Sci Technol, 2015, 52(4): 1881 − 1888. doi:  10.1007/s13197-013-1238-x
    [2] 周琳, 杨柳燕, 李青竹, 等. 西红花栽培、繁育和采后管理研究进展[J]. 中国农学通报, 2020, 36(13): 82 − 88.

    ZHOU Lin, YANG Liuyan, LI Qingzhu, et al. Cultivation, breeding and post-harvest management of Crocus sativus: recent progress [J]. Chin Agric Sci Bull, 2020, 36(13): 82 − 88.
    [3] 钱之玉. 西红花苷调血脂作用的实验和临床研究[J]. 中国执业药师, 2009, 6(2): 6 − 9.

    QIAN Zhiyu. The experiment and clinical study on the hypolipidemic effect of crocin [J]. Chin Licensed Pharm, 2009, 6(2): 6 − 9.
    [4] 胡江宁, 姚德中, 章江生, 等. 西红花抗肿瘤作用的研究进展[J]. 安徽农业科学, 2014, 42(3): 699 − 701, 703. doi:  10.3969/j.issn.0517-6611.2014.03.020

    HU Jiangning, YAO Dezhong, ZHANG Jiangsheng, et al. Research progress of Crocus sativus L. antitumor role [J]. J Anhui Agric Sci, 2014, 42(3): 699 − 701, 703. doi:  10.3969/j.issn.0517-6611.2014.03.020
    [5] 彭海君. 西红花生物学特性、离体快繁及质量评价研究[D]. 杭州: 浙江农林大学, 2014.

    PENG Haijun. Study on Biological Characteristics, In Vitro Propagation and Quality Evaluation of Crocus sativus L. [D]. Hangzhou: Zhejiang A&F University, 2014.
    [6] ZEKA K, MARRAZZO P, MICUCCI M, et al. Activity of antioxidants from Crocus sativus L. Petals: potential preventive effects towards cardiovascular system [J]. Antioxidants, 2020, 9(11): 1102. doi: 10.3390/antiox9111102.
    [7] NEGIN T, MOHSEN M, AMIRHOSSEIN F K, et al. Effects of saffron supplementation on oxidative/antioxidant status and severity of disease in ulcerative colitis patients: a randomized, double-blind, placebo-controlled study [J]. Phytother Res, 2021, 35(2): 946 − 953. doi: 10.1002/ptr.6848.
    [8] LIU Tao, TIAN Li, FU Xuefeng, et al. Saffron inhibits the proliferation of hepatocellular carcinoma via inducing cell apoptosis [J]. Panminerva Med, 2020, 62(1): 7 − 12.
    [9] 刘柏林. 藏红花素对人胰腺癌HPAC细胞凋亡的影响[J]. 中国现代医药杂志, 2016, 18(7): 6 − 9. doi:  10.3969/j.issn.1672-9463.2016.07.002

    LIU Bolin. Effect of crocin on the apoptosis of HPAC cells in human pancreatic carcinoma [J]. Mod Med J China, 2016, 18(7): 6 − 9. doi:  10.3969/j.issn.1672-9463.2016.07.002
    [10] 李梦颖, 司明东, 温子帅, 等. 基于网络药理学的西红花治疗动脉粥样硬化作用机制探讨[J]. 中国临床药理学与治疗学, 2020, 25(6): 649 − 657. doi:  10.12092/j.issn.1009-2501.2020.06.007

    LI Mengying, SI Mingdong, WEN Zishuai, et al. Mechanism of saffron in treating atherosclerosis based on network pharmacology method [J]. Chin J Clin Pharmacol Ther, 2020, 25(6): 649 − 657. doi:  10.12092/j.issn.1009-2501.2020.06.007
    [11] 俞婷, 邢越阳, 朱国琴. 基于网络药理学的西红花抗抑郁作用机制研究[J]. 上海中医药大学学报, 2020, 34(3): 70 − 75.

    YU Ting, XING Yueyang, ZHU Guoqin. Anti-depression mechanism of croci stigma based on network pharmacology [J]. Acad J Shanghai Univ Tradit Med, 2020, 34(3): 70 − 75.
    [12] SHARMA N, NACHANE H, SASIKUMARAN A, et al. Saffron vs sertraline for depression in the elderly [J]. Psychiatry Res, 2020, 285: 112733. doi: 10.1016/j.psychres.2019.112733.
    [13] KOULAKIOTIS N S, PURHONEN P, GIKAS E, et al. Crocus-derived compounds alter the aggregation pathway of Alzheimer’s Disease: associated beta amyloid protein [J]. Sci Rep, 2020, 10(1): 18150. doi: 10.1038/s41598-020-74770-x.
    [14] SUN Chengtao, NILE S H, ZHANG Yiting, et al. Novel insight into utilization of flavonoid glycosides and biological properties of saffron (Crocus sativus L.) flower by-products [J]. J Agric Food Chem, 2020, 68(39): 10685 − 10696. doi:  10.1021/acs.jafc.0c04076
    [15] 吴李芳. 西红花球茎腐烂病的致病菌鉴定及其生防菌解淀粉芽孢杆菌C612的筛选和应用[D]. 杭州: 浙江大学, 2016.

    WU Lifang. Identification of Crocus sativus Corm Rot Pathogenic Fungi and Application on Screening Bacillus amyloliquefaciens C612[D]. Hangzhou: Zhejiang University, 2016.
    [16] 陈健, 孙旭春, 赵庆芳. 渭源县黄芪根腐病病原菌的分离与鉴定[J]. 甘肃农业科技, 2020(10): 21 − 27. doi:  10.3969/j.issn.1001-1463.2020.10.005

    CHEN Jian, SUN Xuchun, ZHAO Qingfang. Identification of pathogens causing root rot of Astragalus membranaceus [J]. Gansu Agric Sci Technol, 2020(10): 21 − 27. doi:  10.3969/j.issn.1001-1463.2020.10.005
    [17] 周双, 白洁, 陈放. 藏红花腐烂病病原真菌的分离鉴定及药剂防治[J]. 四川大学学报(自然科学版), 2015, 52(4): 911 − 916.

    ZHOU Shuang, BAI Jie, CHEN Fang. Identification and fungicides screening of saffrons rot disease pathogen [J]. J Sichuan Univ Nat Sci Ed, 2015, 52(4): 911 − 916.
    [18] CHAMKHI I, ABBAS Y, TARMOUN K, et al. Morphological and molecular characterization of arbuscular mycorrhizal fungal communities inhabiting the roots and the soil of saffron (Crocus sativus L.) under different agricultural management practices [J]. Arch Agron Soil Sci, 2019, 65(8): 1035 − 1048. doi:  10.1080/03650340.2018.1548012
    [19] 张静, 万凌霄, 卢丽珍. 微生物菌剂对重茬地人参根腐病防治效果的研究[J]. 人参研究, 2019(4): 27 − 30.

    ZHANG Jing, WAN Lingxiao, LU Lizhen. Study on the control effect of microbial fertilizer on root rot of Panax ginseng in continuous cropping land [J]. Ginseng Res, 2019(4): 27 − 30.
    [20] RUBIO-MORAGA A, RÁMBLA J L, FERNANDEZ-de-CARMEN A, et al. New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus [J]. Plant Mol Biol, 2014, 86(4/5): 555 − 569.
    [21] FRUSCIANTE S, DIRETTO G, BRUNO M, et al. Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis [J]. Proc Natl Acad Sci USA, 2014, 111(33): 12246 − 12251. doi:  10.1073/pnas.1404629111
    [22] TAN Hexin, CHEN Xianghui, LIANG Nan, et al. Transcriptome analysis reveals novel enzymes for apo-carotenoid biosynthesis in saffron and allows construction of a pathway for crocetin synthesis in yeast [J]. J Exp Bot, 2019, 70(18): 4819 − 4834. doi:  10.1093/jxb/erz211
    [23] 叶军, 张翔, 朱睿睿, 等. 西红花苷生物合成途径研究进展[J]. 中国野生植物资源, 2020, 39(5): 38 − 44, 48. doi:  10.3969/j.issn.1006-9690.2020.05.008

    YE Jun, ZHANG Xiang, ZHU Ruirui, et al. Research progress on biosynthetic pathway of crocin [J]. Chin Wild Plant Resour, 2020, 39(5): 38 − 44, 48. doi:  10.3969/j.issn.1006-9690.2020.05.008
    [24] 李庆, 闫晓剑, 赵魁, 等. 基于云端-互联便携式近红外技术现场快检西红花真伪[J]. 光谱学与光谱分析, 2020, 40(10): 3029 − 3037.

    LI Qing, YAN Xiaojian, ZHAO Kui, et al. Fast inspection of saffron on the spot based on cloud-connected portable near-infrared technology [J]. Spectrosc Spect Anal, 2020, 40(10): 3029 − 3037.
    [25] AMIRVARESI A, RASHIDI M, KAMYAR M, et al. Combining multivariate image analysis with high-performance thin-layer chromatography for development of a reliable tool for saffron authentication and adulteration detection [J]. J Chromatogr, 2020, 1628: 461461. doi: 10.1016/j.chroma.2020.461461.
    [26] DAI Haochen, GAO Qixiang, HE Lili. Rapid determination of saffron grade and adulteration by thin-layer chromatography coupled with raman spectroscopy [J]. Food Anal Methods, 2020, 13(11): 2128 − 2137. doi:  10.1007/s12161-020-01828-x
    [27] LI Shuailing, XING Bingcong, LIN Ding, et al. Rapid detection of saffron (Crocus sativus L.) adulterated with lotus stamens and corn stigmas by near-infrared spectroscopy and chemometrics [J]. Ind Crops Prod, 2020, 152: 112539. doi: 10.1016/j.indcrop.2020.112539.
    [28] CARDONE L, CASTRONUOVO D, PERNIOLA M, et al. The influence of soil physical and chemical properties on saffron (Crocus sativus L.) growth, yield and quality [J]. Agronomy, 2020, 10(8): 1154. doi: 10.3390/agronomy10081154.
    [29] SHAJARI M A, MOGHADDAM P R, GHORBANI R, et al. The possibility of improving saffron (Crocus sativus L.) flower and corm yield through the irrigation and soil texture managements [J]. Sci Hortic, 2020, 271: 109485. doi: 10.1016/j.scienta.2020.109485.
    [30] 陆中华, 朱海燕, 毛碧增, 等. 西红花种球茎异地繁育关键技术研究[J]. 中国现代中药, 2020, 22(4): 573 − 576.

    LU Zhonghua, ZHU Haiyan, MAO Bizeng, et al. Study on key technologies of saffron bulb reproduced in different places [J]. Mod Chin Med, 2020, 22(4): 573 − 576.
    [31] 王梧嵋. 植物病原真菌效应蛋白研究进展[J]. 农业技术与装备, 2020, 368(8): 52 − 54. doi:  10.3969/j.issn.1673-887X.2020.08.022

    WANG Wumei. Studies on effector proteins of plant pathogenic fungi [J]. Agric Technol Equip, 2020, 368(8): 52 − 54. doi:  10.3969/j.issn.1673-887X.2020.08.022
    [32] 王海玲. 西红花花柱离体培养技术及培养物特征特性的研究[D]. 苏州: 苏州大学, 2011.

    WANG Hailing. Study on Technique System of Tissue Culture and Characteristics of Cultures from Style in Crocus sativus L. [D]. Suzhou: Soochow University, 2011.
    [33] 邹凤莲, 汪志平, 卢钢. 番红花链格孢菌的分离及其生物学特性研究[J]. 浙江大学学报(农业与生命科学版), 2006, 32(2): 162 − 167.

    ZOU Fenglian, WANG Zhiping, LU Gang. Studies on biological characteristics of Alternaria Alternata obtained from the corms of Crocus sativus L. [J]. J Zhejiang Univ Agric Life Sci, 2006, 32(2): 162 − 167.
    [34] 张国辉, 张西平, 张年富, 等. 藏红花球茎腐烂病的病原鉴定及药剂预防[J]. 凯里学院学报, 2009, 27(3): 47 − 49. doi:  10.3969/j.issn.1673-9329.2009.03.019

    ZHANG Guohui, ZHANG Xiping, ZHANG Nianfu, et al. Investigation and medicament prevention on stem rot disease of Crocus sativus [J]. J Kaili Univ, 2009, 27(3): 47 − 49. doi:  10.3969/j.issn.1673-9329.2009.03.019
    [35] WANI Z A, AHMAD T, NALLI Y, et al. Porostereum sp., associated with saffron (Crocus sativus L.), is a latent pathogen capable of producing phytotoxic chlorinated aromatic compounds [J]. Curr Microbiol, 2018, 75(7): 880 − 887. doi:  10.1007/s00284-018-1461-9
    [36] ZHANG Tong, HUANG Chao, DENG Changping, et al. First report of corm rot on saffron caused by Penicillium solitum in China [J]. Plant Dis, 2020, 104(2): 579. doi: 10.1094/PDIS-09-19-1927-PDN.
    [37] 姚天明, 伏建增, 南建军, 等. 尖孢镰刀菌及欧文氏杆菌复合侵染引起的半夏腐烂病研究[J]. 甘肃农业科技, 2020(7): 54 − 58.

    YAO Tianming, FU Jianzeng, NAN Jianjun, et al. Study on Pinellia ternata rot caused by combined infection of Fusarium oxysporum and Erwiniella [J]. Gansu Agric Sci Technol, 2020(7): 54 − 58.
    [38] KALHA C S, GUPTA V, GUPTA D, et al. First report of sclerotial rot of saffron caused by Sclerotium rolfsii in India [J]. Plant Dis, 2007, 91(9): 1203. doi: 10.1094/PDIS-91-9-1203B.
    [39] KARUNASINGHE T G, MAHARACHCHIKUMBURA S S N, VELAZHAHAN R, et al. Antagonistic activity of endophytic and rhizosphere fungi isolated from sea purslane (Sesuvium portulacastrum) against pythium damping-off of cucumber [J]. Plant Dis, 2020, 104(8): 2158 − 2167. doi:  10.1094/PDIS-01-20-0003-RE
    [40] HASSANI M A, DURÁN P, HACQUARD S. Microbial interactions within the plant holobiont [J]. Microbiome, 2018, 6: 58. doi: 10.1186/s40168-018-0445-0.
    [41] 郭龙妹, 高林怡, 孙文静, 等. 药用植物内生真菌研究进展[J]. 安徽农业科学, 2019, 47(9): 11 − 13, 18. doi:  10.3969/j.issn.0517-6611.2019.09.004

    GUO Longmei, GAO Linyi, SUN Wenjing, et al. Research progress of endophytic fungi of medicinal plants [J]. J Anhui Agric Sci, 2019, 47(9): 11 − 13, 18. doi:  10.3969/j.issn.0517-6611.2019.09.004
    [42] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979.
    [43] 戴芳澜. 真菌的形态和分类[M]. 北京: 科学出版社, 1987.
    [44] 韩小敏, 李凤琴. 黑曲霉群菌种多相分类和鉴定方法最新研究进展[J]. 食品与发酵工业, 2020, 46(23): 279 − 285.

    HAN Xiaomin, LI Fengqin. Advances in the polyphasic classification and identification method of Aspergillus niger isolates [J]. Food Ferment Ind, 2020, 46(23): 279 − 285.
    [45] 卢丹丹. 北桑寄生内生真菌的分离鉴定及Alternaria alternata次级代谢产物研究[D]. 太原: 山西医科大学, 2020.

    LU Dandan. Isolation, Identification of Endophytic Fungi from Loranthus tanakae and Study on Secondary Metabolites of Alternaria alternate[D]. Taiyuan: Shanxi Medical University, 2020.
    [46] 苟凡铖, 侯怡铃, 宋波, 等. rDNA-ITS序列分析法和传统分类法对真菌进行分类鉴定[J]. 西华师范大学学报(自然科学版), 2017, 38(4): 382 − 386.

    GOU Fancheng, HOU Yiling, SONG Bo, et al. The application of rDNA-ITS sequence analysis method and traditional classification method in the classification of fungi [J]. J China West Norm Univ Nat Sci, 2017, 38(4): 382 − 386.
    [47] 杨明俊, 张琛, 晏祖花, 等. 乌拉尔甘草内生真菌分离及活性初探[J]. 中草药, 2020, 51(17): 4538 − 4546. doi:  10.7501/j.issn.0253-2670.2020.17.023

    YANG Mingjun, ZHANG Chen, YAN Zuhua, et al. Isolation and activity evaluation of endophytic fungi from Glycyrrhiza uralensis [J]. China Tradit Herb Drugs, 2020, 51(17): 4538 − 4546. doi:  10.7501/j.issn.0253-2670.2020.17.023
    [48] 刘慧波, 陈玉婵, 李赛妮, 等. 巴戟天内生真菌Diaporthe lithocarpus的次级代谢产物及其细胞毒活性研究[J]. 中药材, 2020, 43(10): 2439 − 2444.

    LIU Huibo, CHEN Yuchan, LI Saini, et al. Study on the secondary metabolites from endophytic fungus Diaporthe lithocarpus A740 in Morinda offcinalis and the cytotoxic activities [J]. J Chin Med Mater, 2020, 43(10): 2439 − 2444.
    [49] 兰咏哲, 李启瑞, 黄劲, 等. 贵州关岭余甘子内生真菌多样性及其抗菌抗氧化活性[J]. 贵州医科大学学报, 2020, 45(9): 1009 − 1014.

    LAN Yongzhe, LI Qirui, HUANG Jin, et al. Diversity, antibacterial and antioxidant activities of endophytic fungi from Phyllanthus emblica in Guanling, Guizhou [J]. J Guizhou Med Univ, 2020, 45(9): 1009 − 1014.
    [50] 杨涛, 赵疆, 杨晖, 等. 一株强分泌刀孢蜡蚧菌的分离及对甘肃贝母腐烂病防治的研究[J]. 中药材, 2020, 43(11): 2624 − 2630.

    YANG Tao, ZHAO Jiang, YANG Hui, et al. Study on the isolation of strong secretory Lecanicillium psalliotae and its control of rotten disease of Fritillaria przewalskii [J]. J Chin Med Mater, 2020, 43(11): 2624 − 2630.
    [51] 周莹, 吴令上, 陈秋燕, 等. 抗宿主白绢病的铁皮石斛内生真菌的筛选[J]. 中国中药杂志, 2020, 45(22): 5459 − 5464.

    ZHOU Ying, WU Lingshang, CHEN Qiuyan, et al. Screening of endophytic fungi against southern blight disease pathogen-Sclerotium delphinii in Dendrobium catenatum [J]. China J Chin Mater Med, 2020, 45(22): 5459 − 5464.
    [52] WANI Z A, KUMAR A, SULTAN P, et al. Mortierella alpina CS10E4, an oleaginous fungal endophyte of Crocus sativus L. enhances apocarotenoid biosynthesis and stress tolerance in the host plant [J]. Sci Rep, 2017, 7(1): 8598. doi: 10.1038/s41598-017-08974-z.
    [53] ZHENG Chengjian, LI Lin, ZOU Jingping, et al. Identification of a quinazoline alkaloid produced by Penicillium vinaceum, an endophytic fungus from Crocus sativus [J]. Pharm Biol, 2012, 50(2): 129 − 133.
    [54] WANI Z A, MIRZA D N, ARORA P, et al. Molecular phylogeny, diversity, community structure, and plant growth promoting properties of fungal endophytes associated with the corms of saffron plant: an insight into the microbiome of Crocus sativus Linn. [J]. Fungal Biol, 2016, 120(12): 1509 − 1524. doi:  10.1016/j.funbio.2016.07.011
    [55] WEN Lu, XU Yuan, WEI Qiqiu, et al. Modeling and optimum extraction of multiple bioactive exopolysaccharide from an endophytic fungus of Crocus sativus L. [J]. Pharmacognosy Mag, 2018, 14(53): 36 − 43. doi:  10.4103/pm.pm_96_17
    [56] ROMERO D, de VICENTE A, RAKOTOALY R H, et al. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca [J]. Mol Plant Microbe Interact, 2007, 20(4): 430 − 440. doi:  10.1094/MPMI-20-4-0430
    [57] 周洋子. 产脂肽类菌株QHZ-3对马铃薯黑痣病的生防效果及其机理研究[D]. 兰州: 甘肃农业大学, 2020.

    ZHOU Yangzi. Studies on the Biocontrol Effects and Mechanism of Lipopolypeptide Producing Strain QHZ-3 on Black Scurf Disease of Potato[D]. Lanzhou: Gansu Agricultural University, 2020.
    [58] 叶旻硕, 马艳, 黄有军. 生防芽孢杆菌防控辣椒疫病研究进展[J]. 中国农学通报, 2020, 36(15): 123 − 129.

    YE Minshuo, MA Yan, HUANG Youjun. The control of pepper blight by Bacillus spp.: research progress [J]. Chin Agric Sci Bull, 2020, 36(15): 123 − 129.
    [59] 要雅倩, 成娜娜, 李培根, 等. 解淀粉芽胞杆菌Bacillus amyloliquefaciens T-6的分离鉴定及抗病促生潜力[J]. 生物技术通报, 2020, 36(9): 202 − 210.

    YAO Yaqian, CHENG Nana, LI Peigen, et al. Isolation and identification of Bacillus amyloliquefaciens T-6 and its potential of resisting disease and promoting growth [J]. Biotechnol Bullet, 2020, 36(9): 202 − 210.
    [60] KONG H G, KIM N H, LEE S Y, et al. Impact of a recombinant biocontrol bacterium, Pseudomonas fluorescens pc78, on microbial community in tomato rhizosphere [J]. Plant Pathol J, 2016, 32(2): 136 − 144. doi:  10.5423/PPJ.OA.08.2015.0172
    [61] SOOD M, KAPOOR D, KUMAR V, et al. Trichoderma: the “secrets”of a multitalented biocontrol agent [J]. Plants, 2020, 9(6): E762. doi: 10.3390/plants9060762.
    [62] TIAN Lei, SHI Shaohua, JI Li, et al. Effect of the biocontrol bacterium Bacillus amyloliquefaciens on the rhizosphere in ginseng plantings [J]. Int Microbiol, 2018, 21: 153 − 162. doi:  10.1007/s10123-018-0015-0
    [63] 陶中云, 谢国雄, 曾红星, 等. 一株对西红花种球腐烂病有生防效果的蜂房类芽胞杆菌[J]. 植物保护学报, 2013, 40(3): 285 − 286.

    TAO Zhongyun, XIE Guoxiong, ZENG Hongxing, et al. Paenibacillus alvei ZJU20111 as a biocontrol agent against corm rot of saffron caused by Fusarium oxysporum [J]. Acta Phytophylacica Sin, 2013, 40(3): 285 − 286.
    [64] SCHOINA C, STRINGLIS I A, PANTELIDES I S, et al. Evaluation of application methods and biocontrol efficacy of Paenibacillus alvei strain K-165, against the cotton black root rot pathogen Thielaviopsis basicola [J]. Biol Control, 2011, 58(1): 68 − 73. doi:  10.1016/j.biocontrol.2011.04.002
    [65] KOUR R, AMBAROAR S, VAKHLU J. Plant growth promoting bacteria associated with corm of Crocus sativus during three growth stages [J]. Lett Appl Microbiol, 2018, 67(5): 458 − 464. doi:  10.1111/lam.13042
    [66] GUPTA V, KUMAR K, FATIMA K, et al. Role of biocontrol agents in management of corm rot of saffron caused by Fusarium oxysporum [J]. Agronomy, 2020, 10(9): 1398. doi: 10.3390/agronomy10091398.
    [67] GUPTA R, VAKHLU J. Native Bacillus amyloliquefaciens W2 as a potential biocontrol for Fusarium oxysporum R1 causing corm rot of Crocus sativus [J]. Eur J Plant Pathol, 2015, 143: 123 − 131. doi:  10.1007/s10658-015-0670-3
    [68] ANISHA C, RADHAKRISHNAN E K. Gliotoxin-producing endophytic Acremonium sp. from Zingiber officinale found antagonistic to soft rot pathogen Pythium myriotylum [J]. Appl Biochem Biotechnol, 2015, 175: 3458 − 3467. doi:  10.1007/s12010-015-1517-2
    [69] HAN J H, PARK G C, KIM K S. Antagonistic evaluation of Chromobacterium sp. JH7 for biological control of ginseng root rot caused by Cylindrocarpon destructans [J]. Mycobiology, 2017, 45(4): 370 − 378. doi:  10.5941/MYCO.2017.45.4.370
    [70] 姜云, 陈林, 唐浩, 等. 解淀粉芽孢杆菌FG14可湿性粉剂的研制及其对人参锈腐病的田间防治[J]. 吉林农业大学学报, 2020, 42(4): 380 − 385.

    JIANG Yun, CHEN Lin, TANG Hao, et al. Development of Bacillus amyloliquefaciens FG14 WP and field control of ginseng rust rot [J]. J Jilin Agric Univ, 2020, 42(4): 380 − 385.
    [71] 郑明子, 叶海军, 俞仪阳, 等. 生防菌剂“宁盾”对浙贝母的防病促生作用[J]. 中国现代中药, 2020, 22(11): 1871 − 1874.

    ZHENG Mingzi, YE Haijun, YU Yiyang, et al. Effect of a biocontrol agents combination "Ning Dun" on disease prevention and growth promoting of Fritillaria thunbergii [J]. Mod Chin Med, 2020, 22(11): 1871 − 1874.
    [72] 何冬梅, 林婵春, 严铸云, 等. 川芎内生放线菌的分离及根腐病拮抗菌株的筛选[J]. 中药材, 2016, 39(2): 265 − 269.

    HE Dongmei, LIN Chanchun, YAN Zhuyun, et al. Isolation and identification of antagonistic endophytic actinomycetes against root rot disease in Ligusticum chuanxiong [J]. J Chin Med Mater, 2016, 39(2): 265 − 269.
    [73] SONG M, YUN H Y, KIM Y H. Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum [J]. J Ginseng Res, 2014, 38(2): 136 − 145. doi:  10.1016/j.jgr.2013.11.016
    [74] 高芬, 赵晓霞, 闫欢, 等. 黄芪根腐病拮抗芽孢杆菌的筛选鉴定及其对根围细菌群落的影响[J]. 中国中药杂志, 2019, 44(18): 3942 − 3947.

    GAO Fen, ZHAO Xiaoxia, YAN Huan, et al. Screening and identification of antagonistic Bacillus against Astragalus membranaceus root rot and its effect on microorganism community in root zone soil [J]. China J Chin Mater Med, 2019, 44(18): 3942 − 3947.
    [75] VINAYARANI G, PRAKASH H S. Fungal endophytes of turmeric (Curcuma longa L.) and their biocontrol potential against pathogens Pythium aphanidermatum and Rhizoctonia solani [J]. World J Microbiol Biotechnol, 2018, 34: 49. doi: 10.1007/s11274-018-2431-x.
    [76] WANG Qiuxia, SUN Hai, XU Chenglu, et al. Analysis of rhizosphere bacterial and fungal communities associated with rusty root disease of Panax ginseng [J]. Appl Soil Ecol, 2019, 138: 245 − 252. doi:  10.1016/j.apsoil.2019.03.012
    [77] 周琳, 杨柳燕, 蔡友铭, 等. 崇明西红花根际土壤和球茎微生物多样性分析[J]. 核农学报, 2020, 34(11): 2452 − 2459. doi:  10.11869/j.issn.100-8551.2020.11.2452

    ZHOU Lin, YANG Liuyan, CAI Youming, et al. Diversity analysis of microorganism in rhizosphere soil and bulbs of chongming saffron (Crocus sativus L.) [J]. J Nucl Agric Sci, 2020, 34(11): 2452 − 2459. doi:  10.11869/j.issn.100-8551.2020.11.2452
    [78] QIU Fei, ZENG Junlan, WANG Jing, et al. Functional genomics analysis reveals two novel genes required for littorine biosynthesis [J]. New Phytol, 2020, 225(5): 1906 − 1914. doi:  10.1111/nph.16317
    [79] SRINIVASAN P, SMOLKE C. Biosynthesis of medicinal tropane alkaloids in yeast [J]. Nature, 2020, 585: 614 − 619.
    [80] PADDON C J, WESTFALL P J, PITERA D J, et al. High-level semi-synthetic production of the potent antimalarial artemisinin [J]. Nature, 2013, 496: 528 − 532. doi:  10.1038/nature12051
    [81] 陈祥慧. 调控番红花糖苷合成、柱头发育基因挖掘及功能研究[D]. 上海: 中国人民解放军海军军医大学, 2018.

    CHEN Xianghui. Mining and Functional Study of Genes Involved in Crocins Biosynthesis or Stigma Development in Crocus sativus L. [D]. Shanghai: The Second Military Medical University, 2018.
    [82] QIAN Xiaodong, SUN Youping, ZHOU Guifen, et al. Single-molecule real-time transcript sequencing identified flowering regulatory genes in Crocus sativus [J]. BMC Genomics, 2019, 20(1): 857. doi: 10.1186/s12864-019-6200-5.
    [83] 吴丹, 宋爱萍, 史亚东, 等. ‘滁菊’病毒脱除及脱毒苗品质分析[J]. 南京农业大学学报, 2017, 40(6): 983 − 992. doi:  10.7685/jnau.201702024

    WU Dan, SONG Aiping, SHI Yadong, et al. Virus elimination and quality evaluation of virus-free seedlings in Chrysanthemum morifolium‘Chuju’ [J]. J Nanjing Agric Univ, 2017, 40(6): 983 − 992. doi:  10.7685/jnau.201702024
    [84] 王宝霞, 齐永红, 肖雅尹, 等. 半夏茎尖脱毒培养及病毒检测[J]. 植物生理学报, 2018, 54(12): 1813 − 1819.

    WANG Baoxia, QI Yonghong, XIAO Yayin, et al. Virus-free culture and virus detection of stem tip of Pinellia ternata [J]. Plant Physiol J, 2018, 54(12): 1813 − 1819.
    [85] 张晓丽, 李萍, 周彩云, 等. 怀地黄脱毒种苗大田生长性状及产量品质[J]. 植物学报, 2017, 52(4): 474 − 479. doi:  10.11983/CBB16052

    ZHANG Xiaoli, LI Ping, ZHOU Caiyun, et al. Growth characters, yield and quality of virus-free Rehmannia glutinosa seedlings in the field [J]. Chin Bull Bot, 2017, 52(4): 474 − 479. doi:  10.11983/CBB16052
    [86] CHIB S, THANGARAJ A, KAUL S, et al. Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in saffron (Crocus sativus L.) [J]. Plant Methods, 2020, 16: 47. doi: 10.1186/s13007-020-00589-2.
    [87] WANG Kaili, DENG Quanqing, CHEN Jianwen, et al. Physiological and molecular mechanisms governing the effect of virus-free chewing cane seedlings on yield and quality [J]. Sci Rep, 2020, 10: 10306. doi: 10.1038/s41598-020-67344-4.
    [88] 焉兆萍, 李永乐, 赵军, 等. 藏红花内生真菌的分离和代谢产物的初步研究[J]. 上海师范大学学报(自然科学版), 2010, 39(1): 71 − 77.

    YAN Zhaoping, LI Yongle, ZHAO Jun, et al. The initial research on isolation and metabolism of endophytic fungus from Crocus sativus L. [J]. J Shanghai Norm Univ Nat Sci, 2010, 39(1): 71 − 77.
    [89] CASER M, DEMASI S, VICTORINO Í M M, et al. Arbuscular mycorrhizal fungi modulate the crop performance and metabolic profile of saffron in soilless cultivation [J]. Agronomy, 2019, 9(5): 232. doi: 10.3390/agronomy9050232.
  • [1] 尹诗恒, 张绍勇, 刘骕骦, 吴酬飞, 王俊伟, 李阳, 张立钦.  松材线虫侵染对马尾松苗不同部位内生细菌菌群结构的影响 . 浙江农林大学学报, doi: 10.11833/j.issn.2095-0756.20200562
    [2] 周雨苗, 何刚辉, 马绍峰, 邵方雷, 费禹凡, 黄顺寅, 章海波.  土壤微塑料污染的生态效应 . 浙江农林大学学报, doi: 10.11833/j.issn.2095-0756.20200729
    [3] 竹万宽, 许宇星, 王志超, 杜阿朋.  不同生长阶段尾巨桉人工林土壤-微生物化学计量特征 . 浙江农林大学学报, doi: 10.11833/j.issn.2095-0756.20200536
    [4] 赵亚红, 徐翠霞, 马玲, 王彬, 韦赛君, 吕嘉欣, 高岩, 张汝民.  3种常绿树挥发物成分对空气负离子及微生物的影响 . 浙江农林大学学报, 2020, 37(4): 654-663. doi: 10.11833/j.issn.2095-0756.20190521
    [5] 王树和, 周彩勤, 张奎望, 刘慧迪.  金叶女贞棒孢叶斑病菌的生物学特性、致病性及系统发育 . 浙江农林大学学报, 2019, 36(6): 1174-1181. doi: 10.11833/j.issn.2095-0756.2019.06.015
    [6] 白嫆嫆, 高艳明, 李建设, 王兰, 张雪, 刘军丽.  不同营养液配比对营养液膜栽培番茄生长及品质的影响 . 浙江农林大学学报, 2019, 36(6): 1217-1224. doi: 10.11833/j.issn.2095-0756.2019.06.020
    [7] 刘学伟, 王正, 吕全, 张雪萍, 王秀芹, 孔祥波, 张星耀.  落叶松八齿小蠹伴生致病菌的特异性快速检测 . 浙江农林大学学报, 2018, 35(1): 128-134. doi: 10.11833/j.issn.2095-0756.2018.01.017
    [8] 施晓灯, 马履一, 段劼, 桑子阳, 朱仲龙, 姚娜, 周明明, 贾忠奎.  红花玉兰潜在引种气候适生区研究 . 浙江农林大学学报, 2018, 35(4): 705-715. doi: 10.11833/j.issn.2095-0756.2018.04.016
    [9] 尹翠, 孙利鑫, 董艳, 曹震, 张亚红.  根区土壤加温对塑料大棚内红地球葡萄生长发育和品质的影响 . 浙江农林大学学报, 2016, 33(6): 1092-1097. doi: 10.11833/j.issn.2095-0756.2016.06.024
    [10] 吴根良, 郑积荣, 李许可.  不同LED光源对设施越冬辣椒果实品质和产量的影响 . 浙江农林大学学报, 2014, 31(2): 246-253. doi: 10.11833/j.issn.2095-0756.2014.02.013
    [11] 何佩云, 丁贵杰, 谌红辉.  第1代和第2代马尾松林土壤微生物及生化作用比较 . 浙江农林大学学报, 2012, 29(5): 703-709. doi: 10.11833/j.issn.2095-0756.2012.05.011
    [12] 胡玉玲, 胡冬南, 袁生贵, 郭晓敏.  不同肥料与芸苔素内酯处理对5年生油茶光合和品质的影响 . 浙江农林大学学报, 2011, 28(2): 194-199. doi: 10.11833/j.issn.2095-0756.2011.02.004
    [13] 颜晓捷, 黄坚钦, 邱智敏, 努尔阿米娜·热合曼, 朱旻华, 吴家森.  生草栽培对杨梅果园土壤理化性质和果实品质的影响 . 浙江农林大学学报, 2011, 28(6): 850-854.
    [14] 何沙娥, 张智俊.  适用于竹林土壤PCR-DGGE分析用的微生物总DNA提取及纯化方法 . 浙江农林大学学报, 2009, 26(2): 164-168.
    [15] 左继林, 龚春, 汪建平, 周文才, 温强, 徐林初.  赣油茶25个优良无性系品质评价 . 浙江农林大学学报, 2008, 25(5): 624-629.
    [16] 张昕, 张立钦, 林海萍, 张炳欣.  引入黄瓜根围的2株生防菌株的生态效应 . 浙江农林大学学报, 2007, 24(6): 649-653.
    [17] 张昕, 张立钦, 马良进, 林海萍, 毛胜凤, 张炳欣.  生防菌ZJY-1抑菌作用的初步研究 . 浙江农林大学学报, 2007, 24(1): 91-95.
    [18] 孟赐福, 姜培坤, 曹志洪, 徐秋芳, 周国模.  杨梅的硼素营养及施硼技术 . 浙江农林大学学报, 2006, 23(6): 684-688.
    [19] 杨艳红, 陈玉惠, 朱云峰.  西南地区茶生柱锈重寄生菌的分离与鉴定 . 浙江农林大学学报, 2005, 22(4): 414-419.
    [20] 蒋继宏, 陈凤美, 曹小迎, 孙勇, 朱红梅.  银杏内生镰刀菌GI024 生物学特性 . 浙江农林大学学报, 2004, 21(3): 299-302.
  • 加载中
  • 链接本文:

    http://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.20200809

    http://zlxb.zafu.edu.cn/article/zjnldxxb/2021//1

计量
  • 文章访问数:  12
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-01-04
  • 修回日期:  2021-05-20

西红花真菌性病害与生防菌鉴定研究进展

doi: 10.11833/j.issn.2095-0756.20200809
    基金项目:  浙江省自然科学基金面上项目(LY20H280008);全国大学生创新创业训练计划(202010344024)
    作者简介:

    杜雪(ORCID: 0000-0002-8056-7319),从事植物组织培养、致病菌的分离与鉴定研究。E-mail: 1365609131@qq.com

    通信作者: 邵果园(ORCID: 0000-0001-5766-5255),副教授,从事园艺植物新品种选育与营养功效分析研究。E-mail: 360900227@qq.com。周伟(ORCID: 0000-0002-9309-5832),副教授,博士,从事药用植物种质资源创新利用、药效物质代谢合成分子调控和基因组学研究。E-mail: zhouwei19810501@163.com
  • 中图分类号: R282.2

摘要: 西红花Crocus sativus为多年生草本植物,具有活血化瘀、凉血解毒、抗癌、抗氧化等多种药用价值。由于无性繁殖及连作障碍,西红花病害日益严重;以真菌性引起的西红花球茎腐烂病害最为常见,造成西红花产量锐减,严重影响产业发展。解决真菌性病害是提高西红花产量与品质的有效途径之一。围绕西红花土壤真菌性病害、西红花内生真菌、西红花真菌病害生防菌的分离与鉴定研究进展等展开综述,分析西红花研究前景。后续研究可以从3个方向展开,一是基于现代宏基因组测序技术挖掘西红花球茎腐烂致病菌和拮抗菌,二是基于合成生物学和发酵工程技术方法实现西红花药效物质的异源高效生物合成,三是创制西红花脱毒新种质,为生产提供可靠的优质种源。本研究能为西红花真菌性病害防治、生物菌肥的开发利用等研究提供参考。表3参89

English Abstract

杜雪, 李秀娟, 桂思琦, 开国银, 邵果园, 周伟. 西红花真菌性病害与生防菌鉴定研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200809
引用本文: 杜雪, 李秀娟, 桂思琦, 开国银, 邵果园, 周伟. 西红花真菌性病害与生防菌鉴定研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20200809
DU Xue, LI Xiujuan, GUI Siqi, KAI Guoyin, SHAO Guoyuan, ZHOU Wei. Research progress of fungal diseases in Crocus sativus and identification of biocontrol bacteria[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200809
Citation: DU Xue, LI Xiujuan, GUI Siqi, KAI Guoyin, SHAO Guoyuan, ZHOU Wei. Research progress of fungal diseases in Crocus sativus and identification of biocontrol bacteria[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20200809

返回顶部

目录

    /

    返回文章
    返回